相关实验视频

Updated: Jul 17, 2025

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Published on: December 21, 2019

纤维细胞生长因子11通过抑制FXRα抑制抑制B型肝炎病毒基因表达

Mi So Seong¹, Jeong Ah Jang¹, Ye Rim Jeong¹

¹Department of Molecular Biology, Pusan National University, Busan, 46241, Republic of Korea.

Journal of microbiology (Seoul, Korea)

|August 30, 2023

概括

纤维细胞生长因子11 (FGF11) 通过抑制转录因子FXRα来抑制乙型肝炎病毒 (HBV) 基因表达. 这一发现为慢性HBV感染提供了新的治疗点.

科学领域:

分子生物学分子生物学
肝病学肝病学是一种肝病学.
病毒学病毒学

背景情况:

纤维细胞生长因子11 (FGF11) 是细胞内FGF家族的一种成员,其功能尚不清楚.
乙型肝炎病毒 (HBV) 感染仍然是一个重大的全球健康问题,需要新的治疗策略.

研究的目的:

研究FGF11在调节乙型肝炎病毒 (HBV) 基因表达中的作用.
阐明FGF11对HBV复制的影响背后的分子机制.

主要方法:

评估FGF11对HBV基因mRNA和肝细胞中的蛋白质水平的影响.
研究FGF11与核受体FXRα (Farnesoid X受体α) 之间的相互作用.
利用siRNA和主导阴性突变来证实FXRα在FGF11中介抑制中的作用.

主要成果:

在mRNA和蛋白质水平上,FGF11显著降低了HBV基因表达.
FGF11抑制了HBV促进体的FXRα介导的交换活化.
发现FGF11与FXRα相互作用,降低其蛋白质稳定性,并证实对HBV抑制的FXRα依赖性.
FGF11通过抑制肝细胞特异性转录因子FXRα来抑制HBV复制.

结论:

通过FXRα.介导的转录抑制,FGF11对HBV基因表达产生抑制作用.
FGF11与FXRα的相互作用及其后来的稳定性降低是抑制HBV复制的关键机制.
向FGF11-FXRα通路为慢性HBV感染提供了潜在的新型治疗方法,可能通过调节胆酸介导的FXR通路.

关键词:

抗病毒效应抗病毒效应 FGF11 联邦政府基金在 FXR 和 FXR 之间.这是一种HBx蛋白质.乙型肝炎病毒的感染.

更多相关视频

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Published on: June 5, 2020

相关概念视频

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...