m6A介导的circDDIT4生物发生通过隔离ELAVL1/HuR来抑制前列腺癌的进展
Zhe Kong1, Yali Lu1, Yue Yang1
1Obstetrics and Gynecology Hospital of Fudan University, State Key Lab of Genetic Engineering, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Key Laboratory of Reproduction Regulation of NPFPC (SIPPR, IRD), Fudan University, Shanghai, P.R. China.
Molecular cancer research : MCR
|August 30, 2023
概括
循环RNA DDIT4 (circDDIT4) 通过海绵 ELAVL1 / HuR 抑制前列腺癌,减少7 (ANO7) 氨酸胺的表达. N6-甲基氨酸 (m6A) 修改调节circDDIT4水平,影响癌症的进展.
科学领域:
- 分子瘤学分子瘤学
- 在RNA生物学,RNA生物学.
- 癌症基因组学 癌症基因组学
背景情况:
- 圆形RNADDIT4 (circDDIT4) 在前列腺癌中的作用尚不清楚.
- 循环RNA越来越多地被认为是它们在各种疾病 (包括癌症) 中的调节功能.
研究的目的:
- 研究前列腺癌中circDDIT4的功能和调节机制.
- 阐明circDDIT4在前列腺癌进展中的作用及其与其他分子参与者的相互作用.
主要方法:
- 在前列腺癌组织中分析circDDIT4表达.
- 在体外和体内实验中评估circDDIT4的瘤抑制活性.
- 为了研究蛋白质-RNA相互作用,RNA免疫沉和西方斑点.
- 调查N6-甲基氨酸 (m6A) 修饰在circDDIT4生物发生中的作用.
主要成果:
- 在前列腺癌中,circDDIT4被降低调控,并起到瘤抑制作用.
- circDDIT4可以竞争性地与ELAV类RNA结合蛋白1 (ELAVL1/HuR) 结合,从而降低7 (ANO7) 的表达.
- 这种相互作用促进了亡,并抑制了前列腺癌细胞的增殖和转移.
- 由WTAP/METTL3/METTL14和FTO调节的N6-甲基氨酸 (m6A) 修饰,影响circDDIT4生物发生.
结论:
- 在前列腺癌中,circDDIT4通过调节circDDIT4-ELAVL1/HuR-ANO7轴,起到瘤抑制作用.
- 异常的m6A修饰通过循环RNA-蛋白质-细胞信号网络促进前列腺癌.
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