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Updated: Aug 4, 2026

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
11:22

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins

Published on: October 29, 2013

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使用新构造 pGEX4T1/His-T1 的 T1 脂酶净化恢复的增强

Che Haznie Ayu Che Hussian1,2,3, Raja Noor Zaliha Raja Abd Rahman1,2,3, Adam Thean Chor Leow1,2,4

  • 1Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia.

Preparative biochemistry & biotechnology
|August 30, 2023
PubMed
概括

研究人员通过将GST标签替换为聚胺标签来提高热稳定T1脂酶的恢复产量. 这种增强的脂酶净化过程实现了超过98%的回收和工业应用的高特异性活性.

关键词:
利帕斯 (Lipase) 是一种脂肪酶.亲属性染色图谱是一种亲属性染色图.基因工程是基因工程,是基因工程.净化 净化 净化 净化在位点导向的突变发生.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...