在怀孕期间母亲限制和过度养会扰乱绵羊的后代精子表观基因组
Linkai Zhu1, Nicole Tillquist2, Giovanna Scatolin1
1Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, Florida, USA.
Reproduction (Cambridge, England)
|August 30, 2023
概括
怀孕期间母亲的营养对后代产生影响. 孕羊的限制或过度营养并没有改变雄性后代的精液质量,但改变了精子小非编码RNA (sncRNA) 和DNA甲基化,表明了表观遗传效应.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展规划 发展规划
背景情况:
- 孕期母亲的营养对后代的发育和长期健康至关重要.
- 母亲营养状况对男性生殖能力和后代表观遗传特征的影响尚未完全理解.
- 绵羊作为反动物生殖研究的相关模型.
研究的目的:
- 调查孕期母亲受限和过度营养对F1雄性后代羊的精子特征和精子表观遗传学的影响.
- 为了确定怀孕期间母亲的营养挑战是否会导致精子小非编码RNA (sncRNA) 和DNA甲基化模式的改变.
- 探索母体营养,后代表观遗传学和反动物环境影响的表型之间的潜在联系.
主要方法:
- 在怀孕期间控制母亲的饮食摄入量 (60%或140%的NRC要求) 的绵羊模型.
- 对F1男性后代的精液特征 (体积,度,pH,运动性,形态) 和阴囊周长的评估.
- 小RNA测序用于分析F1后代的精子sncRNA概况.
- 全基因组双硫酸盐测序以确定F1后代精子中DNA甲基化中的改变.
主要成果:
- 母亲受限和过度营养没有显著影响F1男性后代的精液特征或阴囊周长.
- 在受限或过度营养的母亲的后代中观察到精子sncRNAs的组成和表达的显著变化.
- 妊娠期间的母体营养干预导致F1男性后代精子中特定基因组位置的DNA甲基化发生变化.
结论:
- 怀孕期间的母体营养影响男性后代的精子表观遗传格局,特别是sncRNA配置文件和DNA甲基化.
- 后代精子中的这些表观遗传修饰可能在调解母亲营养对健康和生殖潜力的长期影响方面发挥作用.
- 这项研究突出了妊娠期间营养编程的潜力,通过绵羊的表观遗传机制影响后代的表型.
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