在O2非热等离子体灭菌后,和聚乙表面的细胞相容性
Christina Maillet1,2,3, Florence M Klein4, Florian Le Bras1
1BIOS EA 4691, Biomatériaux et Inflammation en Site Osseux, SFR CAP Santé, FED 4231, Université de Reims Champagne Ardenne, Reims, France.
PloS one
|August 30, 2023
概括
新的非热等离子体 (NTP) 灭菌是医疗器械的可行替代方案. 这种以氧为基础的NTP工艺与和PEEK材料表现出极好的细胞兼容性,与传统的自闭体和射照射方法相比.
科学领域:
- 生物材料科学 生物材料科学
- 医疗器械灭菌 医疗器械灭菌
- 细胞生物学 细胞生物学
背景情况:
- 医疗器械的灭菌对于预防感染至关重要.
- 传统的方法,如自封式 (AC) 和马辐射 (GI) 不适合某些热或辐射敏感材料.
- 非热等离子体 (NTP) 提供了一个潜在的替代绝育技术.
研究的目的:
- 在使用基于氧的非热等离子体 (O2-NTP) 进行灭菌后,评估 (TA6V) 和聚 (PEEK) 表面的细胞相容性.
- 为了比较O2-NTP绝育与传统的自闭柜 (AC) 和玛辐射 (GI) 方法.
- 评估绝育方法对MG-63骨质细胞样细胞活力,增殖和细胞毒性的影响.
主要方法:
- (TA6V) 和PEEK样品使用AC,GI和O2-NTP进行了灭菌.
- 在消毒表面上培养了MG-63骨质细胞样细胞.
- 用WST-1和DNA量化试验来评估细胞活力和增殖.
- 用乳酸脱酶 (LDH) 试验评估了细胞毒性.
- 通过扫描电子显微镜 (SEM) 检查了细胞粘附和形态.
主要成果:
- 在3-10天后,无论使用哪种灭菌方法,和PEEK材料都提高了细胞活力和增殖.
- 与AC相比,MG-63细胞在GI和O2-NTP后在上表现出更高的生存能力和扩散.
- 在PEEK上,MG-63细胞在O2-NTP治疗后显示出显著更高的生存能力.
- 对于任何一种材料,在两种灭菌方法之间没有观察到扩散的显著差异.
- 在所有物质表面上,SEM证实了成功的细胞殖民.
- 对于任何一种绝育方法,LDH测定表明没有显著的细胞毒性.
结论:
- 氧气非热等离子体 (O2-NTP) 灭菌证明了与和PEEK的自闭体和玛辐射相比较的细胞兼容性.
- O2-NTP是一种有希望的,潜在的成本效益高的杀菌方法,用于与传统方法不相容的脆弱医疗器械.
- 这种技术支持细胞生长和增殖,表明其适用于生物医学应用.
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