在植物中自动提取actin网络
Jordan Hembrow1, Michael J Deeks2, David M Richards1
1Living Systems Institute and Department of Physics and Astronomy, University of Exeter, Exeter, United Kingdom.
PLoS computational biology
|August 30, 2023
概括
我们开发了DRAGoN,这是一个图像分析工具,用于量化植物活性蛋白网络. 这种算法揭示了在各种植物组织和条件中actin属性的显著差异,有助于未来的作物工程.
科学领域:
- 植物细胞生物学 植物细胞生物学
- 细胞骨动力学 细胞骨动力学
- 生物图像分析分析
背景情况:
- 乙细胞骨架对植物生长,发育和防御至关重要.
- 了解植物活性蛋白网络结构-功能关系受到数据分析挑战的限制.
研究的目的:
- 开发一种新的算法,用于对植物活性蛋白网络进行定量分析.
- 为了研究不同植物细胞类型,组织和发育阶段的actin网络配置是如何变化的.
主要方法:
- 开发DRAGoN,这是一个自动化图像分析算法,用于提取actin网络.
- 应用DRAGoN来分析Arabidopsis thaliana在各种组织,突变物和对病原体攻击的反应中的活性网络.
主要成果:
- DRAGoN成功地提取了植物活性网络,并提供了十七种定量测量.
- 在各种实验条件下观察到actin网络属性的统计学上显著差异.
- 该算法证明了适应多种植物样本和生物环境的适应性.
结论:
- DRAGoN提供了一种强大的解决方案,用于量化植物活性蛋白细胞骨动力学.
- 这些发现突显了植物中actin网络组织的上下文依赖性.
- 该算法准备好推进农作物中actin网络的研究和生物工程.
更多相关视频
相关概念视频
Introduction to Actin
5.2K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution. Actin coding genes are conserved within species and across...
5.2K
Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
Actin Polymerization and Cell Motility
5.3K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.3K
Formation of Higher-order Actin Filaments
3.0K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
The high-order actin...
3.0K
Cell Adhesion in Plants
2.7K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.7K
Protein Networks
4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K


