开花抑制器 CmSVP 调用 TOPLESS 核心压缩器来控制花的开花
Zixin Zhang1, Qian Hu1, Zheng Gao2
1State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of State Forestry and Grassland Administration on Biology of Ornamental Plants in East China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
花短植物阶段 (SVP) 蛋白质CmSVP通过抑制CmFTL3表达来延迟开花. 这种抑制需要与TOPLESS (TPL) 核心压缩机相互作用,揭示了一个保存的花抑制机制.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 发育生物学是发展生物学.
背景情况:
- 开花时间对植物繁殖至关重要,并由环境和内部信号调节.
- 这种MADS-box蛋白质的SVP (short vegetative phase) 作用是花过渡的关键抑制剂.
- 通过SVP介导的花抑制的精确转录机制尚未完全理解.
研究的目的:
- 调查花SVP (CmSVP) 在调节开花时间中的作用.
- 阐明CmSVP抑制开花的转录机制.
- 为了确定CmSVP与其他调节蛋白的相互作用.
主要方法:
- 在香中对CmFTL3的基因表达分析.
- 蛋白质与蛋白质相互作用测试以确定CmSVP的相互作用伙伴.
- 在Arabidopsis thaliana进行遗传分析,包括过度表达和突变研究.
- 对SVP和TOPLESS (TPL) 在花和阿拉比多普西斯的同类进行分析.
主要成果:
- 通过与其促进体结合,CmSVP直接抑制了开花基因CmFTL3的表达.
- CmSVP与转录核心压缩剂CmTPL1-2相互作用,形成一种对花压抑至关重要的复合体.
- 在Arabidopsis中证实了SVP-TPL调节模块在抑制开花中的功能性保存.
结论:
- 在花中,CmSVP通过抑制CmFTL3表达而起到花抑制作用.
- SVP-TPL转录复合物被保存,在抑制花过渡方面发挥着关键作用.
- 这项研究揭示了通过与TPL在热传感途径上的相互作用来进行SVP介导的花抑制的保存分子机制.
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