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一种结合性,多药耐药的等离子体的形状依赖的紫外线无活化效率

Achilles Espaldon¹, Kumiko Oguma²

¹Department of Advanced Interdisciplinary Studies, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.

Journal of hazardous materials

|August 30, 2023

概括

紫外线-LED辐射使等离子体上的抗生素耐药性基因失活. 细胞内超的等离子体DNA (pDNA) 由于宿主RNA的光屏蔽,显示出最高的耐药性,影响抗生素耐药性的传播.

科学领域:

微生物学微生物学
分子生物学分子生物学
摄影化学的使用.

背景情况:

结合性等离子体促进了抗生素耐药性基因 (ARG) 的传播.
了解等离子体脱氧核糖核酸 (pDNA) 紫外线失活对于控制ARGs至关重要.

研究的目的:

为了研究紫外线发光二极管 (UV-LED) 基因失活效率在一种多药耐药等离子体上.
为了确定DNA构成和环境 (体内/体外) 对紫外线无活化率的影响.

主要方法:

使用265nm紫外线LED对60kb的RP4等离子体 (IncPα组) 进行辐射.
在大肠杆菌DH5α中的细胞内辐射和在水环境中的细胞外辐射.
实时定量聚合酶连锁反应 (RT-qPCR) 用于测量失活率常量.
紫外线吸收和计算方法来评估光屏蔽和自我屏蔽效应.

主要成果:

对所有测试的等离子体形式观察到的伪第一阶无活化动力学.
细胞内超 pDNA 呈现出最低的紫外线敏感性 (k = 6.1 × 10−3 cm2/mJ).
主体RNA提供了显著的光屏蔽,增加了紫外线抵抗力.
在实验室中,凝聚的DNA对超卷和变质的DNA表现出自我屏蔽的效果.

结论:

大型结合性等离子体的依赖形状的UV/UV-LED无活化是显著的.
紫外线LED技术显示了减轻抗生素耐药性传播的潜力.
了解DNA构成和环境因素是优化基于紫外线的无活化策略的关键.

关键词:

生物纳米材料污染物的污染物.DNA 形状的 DNA 形状.转移和复制基因的DNA和复制基因紫外线消毒的消毒方法紫外线发光二极管 (UV-LED) 是一种

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