水静压缩对小鼠乳腺上皮细胞中与牛奶生产相关的信号通路的影响
Ken Kobayashi1, Liang Han1, Shan-Ni Lu1
1Laboratory of Cell and Tissue Biology, Research Faculty of Agriculture, Hokkaido University, North 9, West 9, 060-8589, Sapporo, Japan.
Experimental cell research
|August 30, 2023
概括
乳腺中的高水静压会通过改变乳腺上皮细胞 (MEC) 中的信号通路来影响乳生产. 压缩可以降低和刺激牛奶蛋白合成,表明直接的调节作用.
科学领域:
- 细胞生物学 细胞生物学
- 哺乳期生物学 哺乳期生物学
- 生物物理学的生物物理.
背景情况:
- 乳腺上皮细胞 (MECs) 在哺乳期间产生牛奶.
- 牛奶的积累会增加乳膜内水静压.
- 高压可能会影响MEC功能和牛奶产量.
研究的目的:
- 研究水静压缩对哺乳期MEC的直接影响.
- 确定压力对牛奶生产信号通路的影响.
主要方法:
- 使用商业压缩装置和MECs的哺乳培养模型.
- 在不同时间段 (1h,8h,重复循环) 应用高水静压 (100kPa).
- 分析了蛋白质水平 (β-casein,claudin-4) 和信号通路活性 (STAT5,葡萄糖皮质体受体,p38 MAPK,Akt,mTOR,STAT3,NF-κB) 的变化.
主要成果:
- 8小时的压缩降低了β-casein,增加了claudin-4和失活了STAT5/葡萄皮质皮质体受体通路.
- 1小时的压缩使STAT5失活,并激活了p38 MAPK信号.
- 每小时重复的压缩激活了阳性 (Akt,mTOR) 和阴性 (STAT3,NF-κB) 乳生产途径,刺激了素和乳酸铁素的产生.
结论:
- 液态压缩直接影响MEC的与牛奶生产相关的信号通路.
- 乳腺内压力变化可以作为乳腺乳生产的直接调节者.
相关概念视频
The Mammary Glands
702
The female breast is a hemispheric projection of variable size positioned anterior to the pectoralis major and serratus anterior muscles. A fascia layer composed of dense, irregular connective tissue connects it to these muscles.
Each breast features a pigmented projection known as the nipple, through which milk emerges via closely spaced openings of ducts, referred to as lactiferous ducts. Surrounding the nipple is a circular pigmented area of skin named the areola, which appears rough due to...
Each breast features a pigmented projection known as the nipple, through which milk emerges via closely spaced openings of ducts, referred to as lactiferous ducts. Surrounding the nipple is a circular pigmented area of skin named the areola, which appears rough due to...
702
Endocrine Signaling
64.4K
Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
64.4K


