提供多价值mRNASARS-CoV-2疫苗的
Cian M McCrudden1, Lindsey Bennie1, Philip Chambers1
1School of Pharmacy, Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK; pHion Therapeutics, Catalyst Concourse Building 2, 20 Queens Road, Belfast BT3 9DT, UK.
概括
一种新的RALA-SME纳米粒子疫苗在临床前模型中显示出强大的免疫性和安全性. 这种基于的平台显示出下一代适应性mRNA疫苗对抗SARS-CoV-2变种的前景.
科学领域:
- 疫苗学 疫苗学 疫苗学
- 纳米技术 纳米技术
- 免疫学 免疫学 免疫学
背景情况:
- 脂质纳米粒子 (LNP) 对mRNA疫苗至关重要.
- 探索替代纳米粒子平台对于推进疫苗技术至关重要.
研究的目的:
- 评估一种新型RALA-SME纳米粒子疫苗的物理化学特性和功能.
- 在临床前模型中评估RALA-SME的免疫性和安全性.
- 为了确定RALA-SME对SARS-CoV-2变种的疗效.
主要方法:
- 使用RALA的Spike, Membrane和Envelope抗原的复合.
- 描述RALA-SME的物理化学特性和抗原封装效率 (>99%).
- 在小鼠和仓鼠中进行皮内注射,随后进行免疫性和安全性评估,并进行病毒中和试验.
主要成果:
- 拉拉-SME表现出99%以上的抗原封装,并诱导出显著的抗原特异性IgG抗体.
- 通过IgG2a:IgG1比率 (>1.2) 和T细胞反应证实了强大的TH1反应.
- 接种疫苗中和了SARS-CoV-2 (武汉-胡-1和Omicron BA.1),防止体重减轻,并抑制了病毒迁移.
结论:
- 拉拉-SME是用于疫苗开发的安全有效的纳米粒子平台.
- 基于的技术允许抗原灵活性,这对于下一代适应性疫苗至关重要.
- 拉拉-SME证明了对SARS-CoV-2变种的广泛保护的潜力.
关键词:
封面抗原是一种抗原.在皮肤内进行皮肤内治疗.膜抗原是一种抗原.这是一种类.拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉这就是SARS-CoV-2病毒.斯派克抗原是一种抗原.疫苗 疫苗 疫苗 疫苗它们是mRNARNA.更多相关视频
10:16Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Published on: August 17, 2022
3.3K
08:07A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
Published on: July 25, 2022
2.6K
相关概念视频
02:50Conjugated Proteins
18.4K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.4K
02:28Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K