热尼斯通过在脂肪细胞中调高尼古丁胺酸转移酶来增强NAD+生物合成
Shun Watanabe1, Riki Haruyama2, Koji Umezawa3
1Division of Food Science and Biotechnology, Graduate School of Science and Technology, Shinshu University, Nagano, Japan.
The Journal of nutritional biochemistry
|August 30, 2023
概括
热尼斯通过激活禁忌素1 (PHB1) 和尼古丁胺酸转移酶 (NAMPT) 来提高脂肪细胞中的尼古丁胺胺二核酸 (NAD+) 水平,改善代谢功能.
科学领域:
- 代谢研究的研究.
- 营养药学科学 营养药学科学
- 分子生物学分子生物学
背景情况:
- 脂肪组织功能障碍,以降低NAD+水平为标志,损害了全身葡萄糖和脂质代谢.
- 开发营养药物来提高脂肪细胞中的NAD+对于代谢健康至关重要.
研究的目的:
- 研究基因斯坦对脂肪细胞NAD+水平的影响,并阐明潜在的分子机制.
- 为了确定参与NAD+生物合成和代谢调节的基因斯坦分子标.
主要方法:
- 基因斯坦治疗脂肪细胞以评估NAD+水平和NAMPT表达.
- 使用基因斯坦固定珠的拉下测试来识别标蛋白质.
- 基因淘汰实验 (Phb1,Nampt) 用于验证分子途径.
- 信号通路 (ERK) 和转录因子活性 (C/EBPβ) 的分析.
- 评估PPARγ酸化和阿迪波内克水平.
主要成果:
- 热尼斯通过上调NAMPT表达来增加NAD+生物合成.
- 禁忌蛋白1 (PHB1) 被确定为一种直接与基因斯坦结合的蛋白质.
- PHB1敲除了基因斯坦对NAMPT和NAD+水平的影响.
- 热尼斯坦通过ERK激活稳定了C/EBPβ,增强了NAMPT转录.
- 热尼斯促进了PPARγ脱化和增加了阿迪波内克丁,这些影响取决于NAMPT和PHB1.
结论:
- 热尼斯有效地增加脂肪细胞的NAD+水平,并恢复代谢功能.
- PHB1是一种新型的标蛋白,通过NAMPT调解基因素的NAD+增强效应.
- 这些发现支持Genistein作为一种NAD+增强营养药的潜力,用于治疗代谢障碍.
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