相关实验视频

Updated: Jul 17, 2025

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Published on: January 12, 2015

鉴定了两个加勒-4蛋白 (PcGal4-L和PcGal4-L-CRD) 以及它们在Procambarus clarkii中AMP表达中的功能

Tingyi Luo¹, Xianfeng Ren², Lixia Fan²

¹Laboratory of Quality and Safety Risk Assessment for Agro-Products of the Ministry of Agriculture (Jinan), Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, 250100, China; College of Marine Science and Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.

Fish & shellfish immunology

|August 30, 2023

概括

这项研究确定了Procambarus clarkia中的两种galectin-4 (PcGal4) 异型,证明了它们通过细菌识别和抗微生物调节在先天免疫力中的关键作用,增强了的免疫抵抗力.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
甲动物生物学甲动物生物学

背景情况:

盖莱克是参与无脊椎动物天生的免疫力和病原体识别的保存莱克.
了解甲类动物中甲素的功能对于水产养殖和疾病管理至关重要.

研究的目的:

描述来自Procambarus clarkia的两种新型加列-4 (PcGal4) 异型.
调查PcGal4在P. clarkia.天生的免疫反应中的作用.

主要方法:

从P. clarkia. clone中克隆PcGal4-L和PcGal4-L-CRD异型体.
对PcGal4异型的遗传学分析.
基因表达分析 (mRNA水平) 作为对细菌挑战 (Aeromonas hydrophila) 的反应.
重组蛋白表达和结合测试 (脂多糖,糖,微生物).
微生物聚合试验.
使用dsRNA和随后的抗微生物 (AMP) 基因表达分析的基因淘汰实验.
在PcGal4中断后的生存率评估.

主要成果:

确定了两个PcGal4异型,PcGal4-L和PcGal4-L-CRD,具有不同的ORF长度和氨基酸组成.
在Aeromonas hydrophila感染后,PcGal4表达在和血细胞中被诱导.
这两种异构体都表现出直接结合细菌成分和广泛的微生物聚合,其中PcGal4-L-CRD表现出更强的结合.
抑制PcGal4导致多个抗微生物基因的表达减少,并降低了P. clarkii的存活率.

结论:

PcGal4在Procambarus clarkia的天生的免疫力中发挥着重要作用.
PcGal4通过细菌识别,结合和调节抗微生物表达来调节免疫反应.
这些发现有助于理解甲类动物免疫抵抗中的甲素功能.

关键词:

一个AMP表达式的表达式.细菌凝结结合是细菌的结合方式.盖莱丁是一种质素.天生的免疫系统天生的免疫系统.这是一种Procambarus clarkii.

更多相关视频

A High-content Assay for Monitoring AMPA Receptor Trafficking

A High-content Assay for Monitoring AMPA Receptor Trafficking

Published on: January 28, 2019

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

Published on: August 8, 2018

相关概念视频

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

Cell Adhesion Molecules - Types and Functions

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...