生物降解的多多巴胺基基基架的制造,表征和潜在的应用,基于天然的大分子分子
Yiyu Wang1, Xinyu Wang2, Xingxun Liu3
1Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou University, Zhejiang, Taizhou 318000, China; Hubei Provincial Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hubei Engineering University, Xiaogan 432000, China.
International journal of biological macromolecules
|August 30, 2023
概括
本研究介绍了用于组织工程的可生物降解氧化藻酸盐 (OSA) 和丝纤维素 (SF) 支架. 聚多巴胺修饰改善了支架特性和药物释放,增强了组织再生.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 聚合物化学 聚合物化学
背景情况:
- 甲基酸盐 (SA) 支架提供缓慢的药物释放,但由于有限的酶降解,生物降解性较差.
- 这限制了它们在生物医学领域和组织工程中的应用.
- 开发具有增强降解和功能性的基于SA的支架至关重要.
研究的目的:
- 设计和合成基于生物降解氧化藻酸盐 (OSA) 的新型支架.
- 为了研究多多巴胺 (PDA) 修改对脚手架性能和性能的影响.
- 评估改造的支架的体外和体外降解,生物相容性和药物释放特征.
主要方法:
- 合成的OSA-SF支架具有不同的混合比率和OSA氧化度.
- 优化了脚手架的组成,并选择了SF/OSA-0.4 (4:1比) 进行进一步的修改.
- 用多多巴胺 (PDA) 修改的SF/OSA-0.4脚手架来创建Dopa/SF/OSA-0.4.4.
- 评估了脚手架的性能,包括稳定性,伸展性,多孔性,热稳定性,血液溶解和细胞毒性.
- 进行了体外和体内生物降解研究.
- 评估组织生长,原形成和药物 (RhB) 释放动力学.
主要成果:
- 开发了可生物降解的SF/OSA支架,其降解速度明显快于SF/SA支架.
- 对SF/OSA-0.4 (Dopa/SF/OSA-0.4) 的PDA修改导致了出色的稳定性,伸展性,多孔结构和热稳定性.
- 多巴/SF/OSA-0.4 呈现出低血解和细胞毒性.
- 虽然PDA修改减缓了体外降解,但体外降解速度明显更快.
- 多巴/SF/OSA-0.4促进了新的组织生长和原结束的形成.
- 修改后的脚手架提高了药物吸收能力,并减少了RhB的突发释放.
结论:
- 与传统的SA支架相比,可生物降解的氧化藻酸盐和丝纤维素支架提供了更好的降解配置.
- 聚多巴胺表面修饰增强了这些支架的物理,化学和生物特性.
- Dopa/SF/OSA-0.4支架表现出优异的生物相容性,促进组织再生,并提供可控的药物释放,使其成为生物医学应用的前景.
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