单克隆重组抗体的高通量净化使用蛋白A涂层膜板系统
Camila Leal-Lopes1, Sara D'Angelo2, M Frank Erasmus2
1New Mexico Consortium, 4200 W. Jemez Rd, Suite 301, Los Alamos, NM 87544, USA.
New biotechnology
|August 30, 2023
概括
一个新的蛋白A膜板系统能够有效净化数百种治疗性单克隆抗体 (mAbs). 这种方法支持快速验证各种疾病的抗体,而无需自动化.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 药物发现 药物发现 药物发现
背景情况:
- 治疗性单克隆抗体 (mAbs) 对于治疗各种疾病至关重要,包括癌症和免疫媒介疾病.
- 高通量发现活动产生了众多潜在的mAb线索,需要有效的净化方法来验证.
研究的目的:
- 实施和评估一种蛋白质-A涂层膜板系统 (PurexaTM AHT) 用于重组 mAbs. 的准备性净化.
- 评估系统的效率和适合下游功能验证许多抗体的有效性.
主要方法:
- 使用PurexaTM AHT膜板系统进行基于蛋白质A的重组mAbs.净化.
- 在不需要自动化系统的情况下进行净化.
- 量化了mAb的回收,并评估了下游测试的适用性.
主要成果:
- 使用PurexaTM AHT系统实现了高效率 (>80%) 的mAb恢复.
- 产生了足够的净化mAbs用于下游选分析,如ELISA和表面等离子体共振 (SPR).
- 证明了数百种重组 mAbs. 的强大净化.
结论:
- 普雷克萨TM AHT膜板系统为准备性 mAb 净化提供了一个强大的,高效的,非自动化的方法.
- 该系统可及时对大量来自发现活动的抗体进行功能验证.
- 实施的方法支持加速治疗抗体的发展.
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