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Dysosmobacter welbionis对葡萄糖,脂质和能量代谢的影响与特定的生物活性脂质有关
Emilie Moens de Hase1, Camille Petitfils2, Mireille Alhouayek3
1Metabolism and Nutrition Research group, Louvain Drug Research Institute (LDRI), UCLouvain, Université catholique de Louvain, Brussels, Belgium; WELBIO-Walloon Excellence in Life Sciences and Biotechnology, WELBIO department, WEL Research Institute, Wavre, Belgium.
Journal of lipid research
|August 30, 2023
概括
一种新型细菌Dysosmobacter welbionis J115T,在食高脂肪饮食 (HFD) 的小鼠中增强了宿主代谢. 它对体重,脂肪量,耐葡萄糖性和与生物活性脂质相关的胰岛素抵抗有积极的影响.
科学领域:
- 微生物学和宿主新陈代谢
- 肠道微生物组和脂管组学
背景情况:
- 高脂肪饮食 (HFD) 诱导代谢功能障碍.
- 肠道细菌Dysosmobacter welbionis J115T显示了可能改善代谢的可能性.
研究的目的:
- 为了研究D. welbionis J115T改善宿主新陈代谢的机制.
- 在体内和体外鉴定和量化D. welbionis J115T产生的生物活性脂质.
主要方法:
- 向性脂组学用于分析细菌培养,小鼠结肠,棕色脂肪组织 (BAT) 和血液中的生物活性脂质.
- 在体外比较D. welbionis J115T和Escherichia coli Nissle 1917之间的脂质生产.
- 对HFD养小鼠的代谢参数,包括体重,脂肪量,葡萄糖耐受性和胰岛素耐药性的评估.
主要成果:
- D. welbionis J115T在被HFD养的小鼠中减少了体重,脂肪增加,改善了葡萄糖耐受性和胰岛素抵抗.
- 在实验室中,D. welbionis J115T产生了19种不同的生物活性脂质,远远超过了E. coli Nissle 1917的产量.
- 代谢改善与血和BAT脂质样本的改变有关,包括C18-3OH的正常化和15-deoxy-Δ12,14-prostaglandin J2的增加,这是一种过氧体增殖器激活受体 (PPAR-γ) 激动体. 在结肠中,溶解素D5水平增加.
结论:
- D. welbionis J115通过产生特定的生物活性脂质,积极影响宿主新陈代谢,其中许多是PPAR-γ激动剂.
- 细菌对宿主新陈代谢的影响与棕色脂肪组织和肠道脂质谱的变化有关.
- D. welbionis J115T显示出作为治疗代谢障碍的益生菌的潜力,并可能改善诸如结肠炎等疾病.
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