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开发用于在宫癌中识别哨戒节点的新型标记剂

Keisuke Kodama¹, Chuya Tateishi², Tsuyoshi Oda²

¹Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

|August 30, 2023

概括

一种新的绿色氨酸 (ICG) 标记物ICG-phytate-liposome (LP) 增强了哨兵淋巴结 (SLN) 保留,以改善淋巴图谱. 这种更安全,更持久的追踪器在妇科癌症手术中表现有前途.

科学领域:

生物医学工程生物医学工程
医疗成像医学成像
纳米技术纳米技术

背景情况:

接近红外线 (NIR) 光成像与内素绿色 (ICG) 对于淋巴细胞映射至关重要.
由于与蛋白质结合,ICG在哨兵淋巴结 (SLN) 中的短暂保留限制了其疗效.
需要改进的追踪器来增强SLN可视化和保留时间.

研究的目的:

开发和评估一种新的光追踪器,ICG-phytate-liposome (LP),用于增强SLN保留.
在临床前模型中评估ICG-phytate-LP的安全性和淋巴行为.
确定ICG-phytate-LP在改善妇科癌症中淋巴结识别方面的潜力.

主要方法:

用最小化的光自灭来准备封装脂体 (LPs) 的氨酸绿 (ICG).
通过将LP与植物酸盐和Ca2+混合,制备了ICG-phytate-LP痕迹剂.
在小鼠和猪中分析了淋巴结保留时间和淋巴流量.
通过猪的死亡率,体重变化和血液测试来评估安全性.

主要成果:

一致大小的LP成功封装稳定ICG.
在ICG-phytate-LP中,粒子大小增加和表面负电荷增加.
在小鼠中,与对照组相比,ICG-phytate-LP在24小时内显示出优异的SLN保留.
在猪中,ICG-phytate-LP显示出良好的安全性,没有副作用或血液参数的显著变化.

结论:

与单独ICG相比,ICG-phytate-LP显著改善了淋巴结保留时间.
在临床前模型中,新型标记剂显示出良好的安全性和有效性.
在妇科癌症外科手术中,ICG-phytate-LP具有作为改善淋巴结识别工具的潜力,特别是在逆关节手术中.

关键词:

印度氨酸绿色的绿色脂质体组是什么? 脂质体组是什么?植物酸酸盐的使用方法护卫淋巴结的护卫淋巴结.追踪器追踪器是一种追踪器.

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