相关实验视频

Updated: Jul 17, 2025

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

一种RNA粘合DNA酶,需要有机溶剂才能发挥作用

Tianjun Chang¹, Guangping Li¹, Dingran Chang²

¹School of Resources and Environment, Henan Polytechnic University, Jiaozuo, 454000, China.

Angewandte Chemie (International ed. in English)

|August 30, 2023

概括

研究人员开发了新的DNAzymes,这些DNAzymes在有机溶剂中发挥作用,如二甲基硫氧化物 (DMSO). 这一突破扩大了催化DNA在非水性环境中的潜在应用.

科学领域:

生物化学生物化学
分子生物学分子生物学
合成生物学合成生物学

背景情况:

DNA酶是催化性DNA分子,通常在水溶液中活跃.
开发用于异常条件的DNA酶,例如有机溶剂,对于扩大它们的应用至关重要.
之前的研究并没有专注于衍生特定需要有机溶剂的DNAzymes的功能.

研究的目的:

隔离和表征需要35%二甲基硫氧化物 (DMSO) 进行催化活动的RNA分裂DNA酶.
为了证明使用强制体内选择来设计DNA酶的有机溶剂依赖功能的可行性.
在非水性系统中扩大催化DNA的实用性和能力.

主要方法:

使用了体外选择和水溶液中的反选择,然后在35%的DMSO中进行了积极选择.
从随机序列DNA池中分离的RNA分裂DNA酶.
描述了衍生DNAzymes的催化活性和金属离子要求.

主要成果:

发现了一种新型的DNA酶,它需要35%的DMSO来产生显著的催化活性,如果没有它,活性就会大大降低.
确定DNA酶需要双价金属离子进行催化,并被单价离子增强.
来自DNAzyme的最小化和更高效的版本.

结论:

高功能的,依赖有机溶剂的DNA酶可以通过强制性体外选择成功分离.
这项工作扩大了DNAzyme在有机溶剂环境中的应用范围.
工程DNA酶为非水性介质中的生物催化和分子工具提供了新的可能性.

关键词:

这就是DNAzyme DNA酶.甲基二甲基硫氧化物甲基二甲基硫氧化物功能性核酸是一种功能性核酸.在体外选择的选择.有机溶剂有机溶剂

更多相关视频

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

相关概念视频

Ribozymes

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Restriction Enzymes

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...