外源的黑激素通过抑制内源的ABA信号来延迟草果的成熟
Sirvan Mansouri1,2, Mahmoud Koushesh Saba3,4, Hassan Sarikhani5
1Department of Horticultural Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
Scientific reports
|August 30, 2023
概括
通过调节生化变化和基因表达,对外应用的黑激素会延迟草果的成熟. 这项研究强调了黑激素.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生化学
- 分子生物学分子生物学
背景情况:
- 果实成熟是一个由荷尔蒙影响的复杂的发育过程.
- 一种植物激素 - - 黑色素 (melatonin) 在各种生理过程中起作用.
研究的目的:
- 为了研究外源性黑激素对草果成熟的作用.
- 分析梅拉托尼治疗后的形态,生化和基因表达变化.
主要方法:
- 草果实在浅绿色阶段注射了0或10μM的黑激素.
- 在15天内收集样本进行分析.
- 评估的参数包括素,可溶性固体,酸度,软化,化合物,酶活性,基因表达,H2O2水平和ABA积累.
主要成果:
- 10微米黑激素治疗导致安东素,可溶性固体和水果颜色的降低.
- 观察到可定位酸度增加,软化和总含量增加.
- 黑素提高了PAL酶活性,DPPH清理能力,以及特定的酸 (甲酸,甲酸,咖啡酸,原酸).
- 延迟成熟与由于NCEDs基因表达减少而抑制的H2O2和ABA水平相关.
结论:
- 外源性黑激素会延迟草果的成熟.
- 黑色素的ROS清理活性与延迟成熟有关.
- 黑素影响关键的成熟路径,包括代谢和ABA信号传递.
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