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对马10分钟和30分钟的甲状腺激素释放激素刺激试验的不精确采样时间的临床影响

Dante M Vorster¹, Wenqing Wang², Kate L Kemp²

¹Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

Equine veterinary journal

|August 31, 2023

概括

甲状腺皮质中介功能障碍 (PPID) 甲状腺释放激素 (TRH) 刺激试验中不精确的采样时间没有显著改变腺上皮质激素 (ACTH) 结果. 然而,诊断分类各不相同,强调了PPID诊断中需要精确的时间和上下文解释的需要.

科学领域:

兽医内分泌学兽医内分泌学
马类内部医学马类内部医学

背景情况:

甲状腺激素释放激素 (TRH) 刺激测试是对马类垂体中介性功能障碍 (PPID) 的诊断工具.
目前的协议涉及10或30分钟的采样时间,由于10分钟协议中的样本采集不准确,可能导致误诊.

研究的目的:

评估不精确的采样时间对30分钟TRH刺激试验协议对诊断PPID的影响.
为了比较10分钟和30分钟TRH测试协议之间的不精确采样的影响.

主要方法:

在15匹对照和12匹PPID马身上进行了体内实验.
血上腺皮质激素 (ACTH) 度在TRH注射后10分钟和30分钟左右的精确和不精确时间点进行测量.
统计分析包括弗里德曼测试,布兰德-阿尔特曼图谱和费舍尔精确测试来评估差异和诊断分类.

主要成果:

不准确的采样导致ACTH度变化,但在协议之间绝对值没有显著差异.
由于采样不准确,PPID的诊断分类在两种方案中的11%的马中发生了变化.
当使用30分钟的协议作为参考时,很少有百分比的马表现出负面或模两可的结果,这可以在实践中被解释为具有临床迹象的积极结果.

结论:

有限的样本大小和马匹之间的变化可能会影响检测ACTH度的微妙差异.
不准确采样对TRH刺激试验的影响在10分钟和30分钟的方案之间没有显著差异.
精确的样本时间和在临床背景下对ACTH度的解释对于准确的PPID诊断至关重要.

关键词:

adrenocorticotropic 激素的使用方法诊断测试试验诊断测试试验诊断测试试验内分泌系统内分泌系统内分泌马的马,马的马,马的马,马的马.拉米尼蒂斯的发病情况 pituitary pars 中间功能障碍 pituitary pars 中间功能障碍

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