对延迟动脉环检测的动脉储存条件的比较
Dylan K McLaughlin1,2, Carson Hoffmann1,2, Maiko Sasaki1,2
1Division of Vascular Surgery, Department of Surgery, Emory University School of Medicine, Atlanta, GA.
JVS-vascular science
|August 31, 2023
概括
优化的储存条件,包括4°C威斯康星 (WI) 溶液和37°C的Opti-MEM,可保护动脉功能进行测试. 血液流动受损,而不是化,影响人体外围动脉疾病 (PAD) 动脉放松.
科学领域:
- 血管生物学 血管生物学
- 生理学 生理学 生理学
- 生物医学工程 生物医学工程
背景情况:
- 动脉环测试对于评估动脉功能至关重要,内皮功能障碍是外围动脉疾病 (PAD) 的关键标志物.
- 标准化ex vivo动脉检测需要优化储存条件,以保持组织活力和功能.
- 以前对储存条件的比较缺乏可靠的验证,特别是在人体动脉中.
研究的目的:
- 为了优化存储条件ex vivo动脉段的内皮细胞 (EC) 测试使用小鼠模型.
- 在各种存储条件下评估人类PAD动脉中的EC功能.
- 假设特定的储存协议将产生优越的动脉功能的保存.
主要方法:
- 在各种条件下 (Opti-MEM在37°C或4°C,Krebs-HEPES,威斯康星溶液在4°C) 保存24小时,并与立即测试进行比较.
- 血管功能通过同度力测试进行评估,测量内皮依赖和独立放松和动脉收缩.
- 人类PAD动脉被测试放松和收缩,分析扰乱与稳定的流量和化的影响.
主要成果:
- 4°C WI和37°C Opti-MEM在小鼠大动脉中保存最好的内皮依赖放松和动脉收缩,与新鲜组织相似.
- 所有测试的储存条件都保持了内皮细胞独立的放松和收缩.
- 在人类PAD动脉中,干扰的流量显著减少了放松 (2%与59%相比),而化没有显著影响. 动脉收缩性可以在更多样本中测量,而不是放松.
结论:
- 在4°C WI或37°C的24小时储存中,Opti-MEM在小鼠模型中有效地保留了关键的动脉功能.
- 在人类PAD动脉中,受损的收获前流量条件损害了ex vivo内皮依赖放松,突出显示了EC功能障碍的持续性.
- 在人类PAD样本中,动脉收缩性比放松性更可靠地保持,流量条件,而不是化,影响放松结果.
相关概念视频
Assessment of blood pressure in brachial artery(two-step method)
757
Measuring blood pressure is a fundamental skill in healthcare that aids in diagnosing and monitoring hypertension and other cardiovascular conditions. An aneroid sphygmomanometer, commonly used in clinical settings, offers a manual and precise method for blood pressure measurement. The technique for using this instrument involves specific steps that must be carefully executed to ensure accuracy. The following detailed description outlines a two-step technique for assessing blood pressure using...
757
Peripheral Arterial Disease II: Clinical Manifestations and Diagnostic Evaluation
17
Clinical manifestationsPeripheral Arterial Disease (PAD) manifests through a range of symptoms, from the characteristic intermittent claudication to atypical presentations and severe complications in advanced stages. Intermittent claudication, a hallmark symptom of PAD, presents as exercise-induced muscle pain that typically resolves within minutes of rest. This pain is reproducible and stems from inadequate blood flow, leading to the accumulation of lactic acid produced during anaerobic...
17
Sites for measruring blood pressure
1.9K
Blood pressure measurement is a fundamental clinical procedure, providing crucial data for assessing cardiovascular health. Among the various sites for this measurement, the brachial and popliteal arteries are predominantly utilized due to their accessibility and the reliability of their readings. This lesson delves into the anatomical significance, methodology, and considerations of measuring blood pressure at these locations.
The Brachial Artery: Primary Site for Blood Pressure Measurement
The Brachial Artery: Primary Site for Blood Pressure Measurement
1.9K
Pre-Procedural Guidelines for Assessing Blood Pressure
595
Accurate blood pressure assessment is crucial for diagnosing and managing various health conditions. To ensure the reliability of these measurements, healthcare professionals must adhere to standardized pre-procedural guidelines. These guidelines enhance patient safety and improve the overall quality of healthcare. The following steps are essential for obtaining accurate and consistent blood pressure readings, from using the appropriate tools to ensuring effective communication with the...
595


