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溶性铁增强了Shewanella oneidensisMR-1的细胞外电子吸收

Karla Abuyen¹, Mohamed Y El-Naggar^1,2,3

¹Molecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089.

ChemElectroChem

|August 31, 2023

概括

溶性铁可以增强微生物从阴极吸收到Shewanella oneidensis MR-1的电子. 这一过程涉及铁作为电子载体作用于细菌的细胞外电子转移 (EET) 机制.

科学领域:

微生物学微生物学
生物化学生物化学
电化学电化学电化学

背景情况:

微生物利用细胞外电子转移 (EET) 与外部氧化还原化合物相互作用.
对细胞从外部捐赠者接受电子的内向EET的理解仍然有限.
目前的知识集中在通过细胞染色体和氧化还原穿介导的外向EET上.

研究的目的:

调查可溶铁在促进Shewanella oneidensis MR-1进入的EET中的作用.
阐明铁对阴极电子吸收的影响机制.
为了确定涉及铁介导ETE的微生物成分.

主要方法:

使用Shewanella oneidensis MR-1生物膜在阴极上的电化学实验.
不同的外源铁 (FeCl2) 度以评估剂量依赖的影响.
使用铁化剂来确认铁的作用.
使用烟酸作为细胞内电子受体.
比较缺乏特定细胞染色体的野生型和突变菌株.

主要成果:

溶性铁 (FeCl2) 显著增强了从阴极到S.oneidensis的电子吸收.
这种依赖铁的电子吸收被铁化剂取消.
该过程要求存在烟酸还原酶,表明其参与.
缺少外膜和周等离子体细胞染色体的突变体显示电流减少,这表明它们在途径中的作用.
铁度与电子吸收率直接相关.

结论:

溶性铁作为电子载体,促进从阴极到S.oneidensis的内向EET.
包括烟酸还原酶和特定细胞染色体在内的ETE机制对于这一过程至关重要.
这一发现扩大了对微生物电子吸收机制的理解.

关键词:

谢瓦内拉 (Shewanella) 是一个美女.电子吸收的电子吸收.这是电合成的电解.细胞外电子转移细胞外电子转移微生物的电化学.

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