通过IGF-1对工程带结构和功能的拉巴胺素不敏感的调节
El Sayed El Essawy1,2, Keith Baar2,3,4
1Department of Sport Psychology, Mansoura University, Mansoura, Egypt.
Journal of applied physiology (Bethesda, Md. : 1985)
|August 31, 2023
概括
胰岛素样生长因子-1 (IGF-1) 通过减少独立于mTORC1.1.的原体分解来增强工程带强度. 然而,Rapamycin阻断了IGF-1对原蛋白合成的益处,这可能解释了康复方面的挑战.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 整形外科手术 整形外科手术
背景情况:
- 前十字带 (ACL) 破裂需要手术修复,因为自然愈合不良.
- 工程带移植目前缺乏足够的强度用于临床使用.
- 胰岛素样生长因子-1 (IGF-1) 在改善工程带特性方面显示出有前途.
研究的目的:
- 研究mTORC1途径在IGF-1介导的改进工程人类带中的作用.
- 确定IGF-1对原蛋白含量和机械性质的影响是否取决于mTORC1活动.
- 阐明IGF-1增强工程带质量的机制.
主要方法:
- 工程人类带被用IGF-1和/或拉巴胺素 (一种mTORC1抑制剂) 治疗.
- 在治疗后评估了机械性能 (强度,刚性) 和原蛋白含量.
- 量化了原蛋白合成和原蛋白降解率.
主要成果:
- IGF-1显著增加了机械性能 (34%) 和原蛋白含量 (63%).
- 拉巴胺素降低了机械性能 (-24.5%) 和原蛋白含量 (-36%).
- 独立于拉巴胺素,IGF-1降低了原蛋白降解 (15%),而拉巴胺素则阻断了IGF-1对原蛋白合成的影响.
结论:
- IGF-1 增强工程带机制和原蛋白含量,主要是通过通过mTORC1-独立的途径减少原蛋白降解.
- 虽然IGF-1的抗代谢作用是mTORC1独立的,但其对原蛋白合成的合成作用被拉巴素阻断.
- 这些发现表明IGF-1,mTORC1和原代谢之间的复杂相互作用可能会影响ACL移植的愈合和康复.
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