银离子色谱可以将二甲基基化醇从基化醇分离出来
Tim Hammerschick1, Walter Vetter1
1Department of Food Chemistry (170b), Institute of Food Chemistry, University of Hohenheim, Stuttgart, Germany.
Journal of separation science
|August 31, 2023
概括
一种新的银离子色谱法完全从谷物中分离出基醇 (ARs) 和轻微的2-甲基-5-基醇 (mARs). 这一进展使得在小麦和麦中检测到以前未经表征的生物活性化合物.
科学领域:
- 脂组学和天然产品化学
- 食品化学和分析与分析.
背景情况:
- 基醇 (ARs) 是一种多样化的脂质,主要存在于小麦和麦等谷物中.
- 这些化合物包括5-基复合 (ARs) 和研究较少的2-甲基-5-基复合 (mARs),其基链长度和替代剂不同.
- 准确分离和识别ARs和mARs对于理解它们的生物作用至关重要.
研究的目的:
- 开发一种方法,使和的2 - 甲基-5 - 基酸盐 (mARs) 从常规基酸盐 (ARs) 完全分离出来.
- 描述小麦和华提取物中ARs的结构多样性,包括小的mARs.
- 为了证明银离子染色学 (SIC) 对于基于基链替代剂的脂质分离的实用性.
主要方法:
- 从小麦和菜提取物中缩ARs,使用固态提取.
- 应用银离子染色学 (SIC) 在浸AgNO3的凝上,以分离和mARs和常规ARs.
- 气色谱/质谱 (GC/MS) 用于检测和识别分离的ARs.
主要成果:
- 一种新的SIC方法实现了和mAR和传统AR的前所未有的分离.
- 和的mARs使用特定的溶剂系统 (n-hexane / ethyl acetate, 92: 8v / v) 化,而常规的ARs使用不同的系统 (80: 20v / v) 化.
- 在GC/MS分析中,在小麦中发现了35个AR (包括5个mAR),在麦中发现了45个AR (包括21个mAR),发现了几种稀少且可能未知的化合物.
结论:
- 开发的SIC方法提供了基于基链和和替代模式的基复合醇的有效分离.
- 这种技术显著提升了谷物谷物中复杂的AR配置文件的表征.
- 这些发现扩大了已知的基醇多样性,包括以前研究不足的mARs,在主食来源中.
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