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预分析和分析因素对六种调节性血清蛋白质量化的影响

Felix Menne¹, Nicolas A Henzen², Marc Sollberger²

¹Predemtec AG, Rudower Chaussee 29, 12489, Berlin, Germany.

|August 31, 2023

概括

人类血清中的蛋白质度因采血管材料和使用的免疫测试平台而有显著差异. 这些差异影响生物标志物测量,突显了实验室诊断标准化的必要性.

科学领域:

生物化学生物化学
临床化学临床化学
实验室医学实验室医学

背景情况:

人类血清中的蛋白质度分析对于各种诊断和研究应用至关重要.
蛋白质测量的变化可能来自分析前的因素,包括样本采集和处理.
方法的标准化对于可靠和可重复的生物标志物量化至关重要.

研究的目的:

研究不同采血管材料 (玻璃与聚烯) 对血清蛋白度的影响.
评估不同免疫测试平台对特定蛋白质生物标记物的测量影响.
量化变异性并确定由管类型和测试平台引入的蛋白质特异性差异.

主要方法:

在玻璃和聚烯管中收集了292名参与者的血清样本.
六种蛋白质 (BDNF,IGF-1,VEGF-A,TGF-β1,MCP-1,IL-18) 的度使用酶相关免疫吸收试验 (ELISA) 进行测量.
此外,在一个新的自动免疫测试平台上,除IGF-1 (n=211),还对所有蛋白质进行了测量,并使用布兰德-阿尔特曼分析进行了变性评估.

主要成果:

管道材料的比较显示,不同蛋白质的平均偏差范围从-0.45%到-70.64%.
免疫测试平台的比较显示平均偏差在21.04%和-128.10%之间.
在管和平台比较中观察到显著的蛋白质特异性变化.

结论:

血清蛋白度受到血液采集管材的选择的显著影响.
使用的免疫测试平台对蛋白质定量结果有很大影响.
这些发现强调了考虑分析前变量和测试平台的关键需求,以进行准确和一致的生物标志物分析.

关键词:

阿尔茨海默病的生物标志物艾丽莎·伊丽莎·伊丽莎血清是血液中的血清.诊断诊断诊断诊断诊断免疫测试是一种免疫测试.方法比较方法比较

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