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在液体生物配制中有益微生物的生存和使用RSM技术优化不同载体材料的优化

Sayma Serine Chompa¹, Ali Tan Kee Zuan², Adibah Mohd Amin¹

¹Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

International microbiology : the official journal of the Spanish Society for Microbiology

|August 31, 2023

概括

这项研究开发了一种具有成本效益的液体生物肥料,使用耐盐细菌来改善盐土中的大米生产. 树脂显著增强了细菌的生存,为可持续农业提供了一个有希望的解决方案.

科学领域:

农业科学农业科学
微生物学微生物学
生物技术是生物技术.

背景情况:

土壤盐度是湿地大米种植的主要限制,降低了产量和质量.
耐盐植物生长促进根茎细菌 (PGPR) 显示出减轻度压力和增强作物生长的潜力.
开发稳定的液体生物肥料配方对于PGPR的实际应用至关重要.

研究的目的:

开发一种使用多菌株盐耐受性PGPR的货架稳定的液体生物肥料配方.
为了优化配方,使用糖醇和三醇等添加剂来延长细菌的保质期.
评估优化配方对增强PGPR存活率的疗效.

主要方法:

对耐盐性PGPR菌株 (Bacillus tequilensis,Bacillus aryabhattai) 的隔离和选择.
配方开发使用糖醇,三糖和聚烯利 (PVP).
使用殖民地形成单位 (CFU/mL) 的保质期研究和种群分析.
使用响应表面方法和设计专家软件进行优化.

主要成果:

10毫米的醇显著提高了混合PGPR菌株的保质期和存活率.
优化的配方在3个月后实现了9.73×10^7 CFU/mL的细菌种群.
响应表面方法验证了细胞密度和配方稳定性的最佳条件.
优化配方的精度比率超过了99.7%.

结论:

成功开发了一种具有成本效益的,以三糖醇为基础的液态生物肥料配方.
该配方确保了耐盐性PGPR的长期存活,使其适合商业用途.
这项创新提供了一种可持续的方法来对抗水栽培中的土壤盐度.

关键词:

添加剂添加剂添加剂生物配方生物配方生物配方这是PGPR的PGPR.响应表面的方法响应表面方法货架寿命货架寿命

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