高脂肪饮食对与年龄相关的耳频率重新调度的不同结果
Yu Zhang1,2,3, Guotong Lin1,2,3, Na Xue1,2,3
1Department of Otolaryngology, Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
概括
高脂肪饮食可以通过保持外皮毛细胞功能和耳频率编码来保护特定小鼠菌株免受与年龄相关的听力损失. 这表明饮食,遗传学和听力健康之间存在联系.
科学领域:
- 听觉神经科学 听觉神经科学
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 听觉频率编码依赖于基底膜外毛细胞 (OHC) 构造膜网络的机械性质.
- 外发细胞 (OHC) 电动性,由普雷斯介导,增强耳频率选择性和灵敏性.
- 听力损失是与年龄相关的听力损失 (ARHL) 和神经感官听力损失的主要原因.
研究的目的:
- 研究高脂肪饮食 (HFD) 对不同遗传背景的ARHL的影响 (C57BL/6J和CBA/CaJ).
- 为了确定遗传背景和代谢状态是否可以在HFD暴露的情况下保留耳频率位置编码.
- 阐明了基于饮食诱导的抗ARHL保护的分子机制.
主要方法:
- 在C57BL/6J (B6) 和CBA/CaJ (CBA) 小鼠中对ARHL的HFD影响的比较研究.
- 使用扭曲产物耳声发射 (DPOAE) 组延迟测量对耳功能进行评估.
- 分析Prestin水平,OHC形态和耳基因表达 (RNA-seq),专注于TRPV1和脂质平衡.
主要成果:
- 在老年B6小鼠食HFD时,观察到对ARHL的显著保护作用,更好地维持Prestin水平和OHC大小.
- 频率位置编码,以保存的DPOAE群延迟表示,在B6 HFD组中保持稳定.
- 在CBA小鼠中,HFD对听力值的益处很小,群体延迟随年龄而缩短,与B6 HFD队列不同.
- 耳RNA-seq揭示了TRPV1的差异表达和改变的脂质平衡作为对HFD的反应.
- 在B6 HFD小鼠中,TRPV1的激活和减少的桃酸与下调的炎症反应相关,保护耳.
结论:
- 遗传背景和代谢状况显著影响尾虫对HFD的反应,影响ARHL.
- 高频传感器可以保留耳频率位置编码,并改善敏感遗传菌株的与年龄相关的音调感知 (B6).
- 在B6小鼠中,TRPV1的激活和脂质代谢的调节是保护尾细胞免受HFD诱导的ARHL的关键机制.
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