在患有炎症性肠病患者的粘菌相关微生物群中,直肠和组织样本之间的关系
Tsunglin Liu1, Cheng-Han Lin2, Yi-Lin Chen3
1Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan.
Inflammatory bowel diseases
|August 31, 2023
概括
直肠扫描提供了一种不那么侵入性的方法来评估炎症性肠病 (IBD) 患者的肠道微生物群. 虽然拭子微生物群与便微生物群相比,更像组织微生物群,但仍然存在明显的差异,肠道制备改变了组成.
科学领域:
- 胃肠病学 胃肠病学
- 微生物组研究 微生物组研究
- 分子生物学分子生物学
背景情况:
- 与肠道粘膜相关的微生物群与疾病表型相关,但组织活检是侵入性的.
- 直肠拭子是微生物群分析的活检的一个潜在的,不那么侵入性的替代品.
- 在炎症性肠道疾病 (IBD) 中,直肠扫描在代表肠道微生物群的有效性受到争议.
研究的目的:
- 评估IBD患者结肠组织,直肠拭子和便样本之间的微生物群组成差异.
- 为了比较直肠片微生物群与结肠组织微生物群与便微生物群的相似性.
- 评估肠道制备对直肠拭子微生物群的短期影响.
主要方法:
- 收集了来自IBD和对照患者的结肠活检组织,直肠拭子和便样本.
- 使用β多样性和属丰度分析微生物组成.
- 从一小部分IBD患者中收集了准备前的直肠拭子,以评估肠道准备效果.
主要成果:
- 在所有群体中,便微生物群的组成显著不同于抽样和组织微生物群.
- 片微生物群与便微生物群相比,与组织微生物群更为相似,尽管差异仍然存在.
- 短时间的肠道准备并没有改变整体的微生物群,但导致了个体基因组合的明显变化.
结论:
- 在IBD患者中,直肠拭子提供了一个与结肠组织相似的微生物群样本,而不是便样本.
- 尽管有相似之处,直肠拭子并不能完全复制与组织相关的微生物群.
- 肠道制备对直肠拭子微生物群的影响在个体属层面上是显著的,尽管没有总体变化.
相关概念视频
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
354
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
354
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
213
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
213
01:17Chronic Bowel Disorders: Introduction
485
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
485
01:30Inflammatory Bowel Disease II: Crohn's Disease
285
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
285