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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 17, 2025

Single-Molecule Imaging of Nuclear Transport
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使用高分辨率激光扫描共聚焦显微镜成像和量化核孔综合体的协议.

Jocelyn D Mich-Basso1, Bernhard Kühn1

  • 1Division of Cardiology, Pediatric Institute for Heart Regeneration and Therapeutics (I-HRT), UPMC Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15224, USA; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA.

STAR protocols
|August 31, 2023
PubMed
概括

这项研究提供了一种用于图像和量化核孔复合体 (NPC) 的协议,这些复合体对细胞通信和染色质组织至关重要. 该方法使用免疫光和共聚焦显微镜来准确计算细胞中的NPC数量.

关键词:
细胞生物学 细胞生物学显微镜的使用方法

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Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays
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Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
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Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

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Last Updated: Jul 17, 2025

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Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays
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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 显微镜的使用方法

背景情况:

  • 核孔复合体 (NPC) 是核细胞质运输的重要通道.
  • 在调节基因表达和染色体组织方面,NPCs起着至关重要的作用.
  • 准确量化NPC对于了解细胞过程至关重要.

研究的目的:

  • 介绍细胞中核孔综合体的成像和量化详细方案.
  • 为研究NPC动态和功能的研究人员提供可复制的方法.

主要方法:

  • 细胞培养和涂层.
  • 为NPC蛋白质进行免疫光染色.
  • 对焦显微镜用于3D可视化.
  • 数字值与人类监督进行量化.

主要成果:

  • 该协议能够精确地对细胞结构内的NPC进行成像.
  • 通过监督数字分析方法来实现NPC数量的量化.
  • 该方法允许对NPC分布进行详细的3D数据分析.

结论:

  • 该协议提供了一种可靠的方法来量化核孔综合体.
  • 提出的技术有助于研究NPC在细胞通信和染色质结构中的作用.