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微生物群的条件是肠道环境选择野生类型的沙门氏菌Typhimurium毒性
Ersin Gül1, Erik Bakkeren1,2,3, Guillem Salazar1,4
1Institute of Microbiology, Department of Biology, ETH Zurich, Zurich, Switzerland.
PLoS biology
|August 31, 2023
概括
肠道微生物群可以通过清除不那么有毒的突变物来维持沙门氏菌Typhimurium的毒性. 健康的微生物群可以防止这些突变的出现,从而保护病原体.
科学领域:
- 微生物学 微生物学
- 肠道微生物组研究研究
- 传染病的动态传染病的动态.
背景情况:
- 沙门氏菌Typhimurium通过HildD控制的毒性因子引起肠道炎症,从而损害殖民抵抗力 (CR) 并促进病原体的生长.
- 矛盾的是,炎症可以选择有毒的hild突变物,这引发了关于自然孤立物中野生类型毒性的维持的问题.
研究的目的:
- 调查支持维持沙门氏菌Typhimurium毒性的条件.
- 确定肠道微生物群在保护毒性S. Typhimurium基因型中的作用.
主要方法:
- 利用了具有多样化微生物群组成和抗生素或炎症诱导的干扰的小鼠模型.
- 从未感染或已康复的主体采用微生物群转移实验.
- 分析了短暂与不可逆转的微生物群破坏对hild突变动态的影响.
主要成果:
- 从健康捐赠者的微生物群转移迅速清除了减弱的hild突变,保留了毒性S. Typhimurium基因型.
- 不可逆转的微生物群破坏导致了hilD突变的积累,而暂时的破坏允许恢复.
- 重生微生物群,特别是Lachnospirales和Oscillospirales,在短暂的破坏后阻止了hilD突变选择.
结论:
- 过渡性和受控的微生物群扰乱有利于S. Typhimurium,允许病原体的增殖,并最大限度地减少毒性损失.
- 肠道微生物群在维持肠病原体的毒性方面发挥着至关重要的作用,除了赋予殖民抵抗力外.
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