在巴斯综合征的急性运动中,肝脏葡萄糖分解的升高调节了血糖的升高
George G Schweitzer1, Grace L Ditzenberger2, Curtis C Hughey3
1Center for Human Nutrition, Washington University School of Medicine, St. Louis, Missouri, United States of America.
PloS one
|August 31, 2023
概括
带有巴斯综合征 (BTHS) 突变的小鼠在运动期间表现出肝脏糖原分解的增加,支持更高的无氧代谢. 这项研究量化了Tafazzin knockdown小鼠在休息和运动期间的肝脏葡萄糖产量.
科学领域:
- 生物化学 生化学
- 遗传学 是一个遗传学.
- 身体生理学 身体生理学
背景情况:
- 巴斯综合征 (BTHS) 是一种由塔法津 (TAFAZZIN) 基因突变引起的X相关疾病,导致心脏和骨肌肉中的线粒体功能障碍.
- 由于肌肉氧化代谢受损,BTHS患者在运动期间对无氧代谢的依赖性增加.
- 塔法津缺乏对运动期间肝脏葡萄糖产生的影响尚不清楚.
研究的目的:
- 为了研究塔法津缺乏在运动期间肝脏葡萄糖生产中的作用.
- 为了量化体内肝脏葡萄糖生成和糖原分解在休息和运动期间的Tafazzin淘汰小鼠.
主要方法:
- 雄性Tafazzin shRNA转基因 (TG) 和野生类型 (WT) 老鼠接受了详尽的跑步机运行.
- 稳定同位素输注 (2H和13C) 用于量化肝脏葡萄糖生产和休息和运动期间的营养流.
- 分析了血代谢物和组织样本,以评估代谢概况.
主要成果:
- 塔法兹林敲击小鼠在运动后表现出较低的运动耐受性和较高的血乳酸盐度.
- 尽管静止时的动脉葡萄糖水平相似,但TG小鼠在运动期间血糖水平较高,这是由于肝脏葡萄糖分解增加所致.
- 葡萄糖生成和TCA循环流量是可比的,但在运动期间,TG小鼠的乳酸脱酶流量趋势较高.
结论:
- 在Tafazzin敲击下来的小鼠中,肝脏葡萄糖分解的升高支持在运动期间观察到的无氧代谢增加.
- 这些发现为巴斯综合征中的代谢适应提供了洞察力.
相关概念视频
01:02Glucose Homeostasis: Regulation of Blood Glucose
1.7K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
1.7K
01:18Metabolic States of the Body: The Postabsorptive State
360
The postabsorptive state usually starts about four hours after a meal and lasts until the next meal is eaten. During this time, the digestive system stops absorbing nutrients, and the body uses stored energy reserves to maintain stable blood glucose levels.
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
360
01:15Hypoglycemia and Glucagon
295
Without prolonged fasting, healthy individuals maintain blood glucose levels above 3.5 mM due to a well-adapted neuroendocrine counterregulatory system that effectively prevents acute hypoglycemia, a potentially life-threatening condition. The primary clinical scenarios for hypoglycemia encompass diabetes treatment, inappropriate production of endogenous insulin or insulin-like substances by tumors, and the use of glucose-lowering agents in non-diabetic individuals. Notably, hypoglycemia in the...
295
01:16Hormones Regulating Blood Glucose
3.5K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
3.5K
01:19Overview of Carbohydrate Metabolism
1.1K
Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
1.1K
01:24Metabolic States of the Body: Fasting and Starvation
1.5K
During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...
1.5K