全基因组适应性分析揭示了细菌用来与Trichoderma atroviride外代谢物相互作用的分子机制
José Manuel Villalobos-Escobedo1,2, Maria Belen Mercado-Esquivias1,2, Catharine Adams1,2
1Plant and Microbial Biology Department, The University of California, Berkeley, California, United States of America.
PLoS genetics
|August 31, 2023
概括
的排泄物通过营养竞争和抗微生物抗性机制对土壤细菌产生影响. 意想不到的是,这些排泄物也提供了必需的 purin,揭示了复杂的有益和敌对关系.
科学领域:
- 微生物生态学 微生物生态学
- 植物与微生物的相互作用
- 的二次代谢物 的二次代谢物
背景情况:
- 三皮菌 spp. 三皮菌. 是常见的土壤真菌,产生影响植物相关微生物组的代谢物.
- 在根球中调解细菌-真菌相互作用的机制尚未完全理解.
- 了解这些相互作用对于农业应用和土壤健康至关重要.
研究的目的:
- 在Trichoderma atroviride排泄物存在时,识别对健康至关重要的细菌基因.
- 阐明细菌对真菌代谢物反应中的特定机制 (例如,铁竞争,抗菌素耐药性).
- 调查Hog1-MAP激酶在T. atroviride代谢产生的作用及其对细菌的影响.
主要方法:
- 使用随机条码转位子位点测序 (RB-TnSeq) 来选细菌突变库.
- 选择的树叶球细菌 (Klebsiella michiganensis,Herbaspirillum seropedicae,Pseudomonas simiae) 和一个不是树叶球的物种 (Pseudomonas putida).
- 在真菌排泄物上进行了代谢分析,并评估了细菌辅食性突变性救援.
主要成果:
- 固定的细菌争夺铁,需要TonB/ExbB系统来适应T. atroviride排泄物.
- P. simiae和P. putida依赖于膜脂质的修饰,以抵抗阴离子抗微生物 (CAMPs).
- 包括来自Δtmk3突变的T.atroviride排泄物,意外地拯救了纯素辅型细菌突变,表明纯素和复杂代谢物分泌.
结论:
- 三体的代谢物对土壤细菌具有敌对 (铁竞争,CAMP) 和有益 (纯素提供) 的作用.
- 在T. atroviride中的Hog1-MAP激酶 (Tmk3) 影响某些二次代谢物的产生,包括那些影响细菌健康的代谢物.
- 这项研究突出了Trichoderma真菌和根球细菌之间的复杂,多方面的相互作用,影响了微生物组的动态.
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