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微生物用于无标签检测短并列重复:对缓解次要结构效应的洞察

Omair Adil1, Seth B Eddington2, Keith T Gagnon1,2

  • 1School of Chemical and Biomolecular Sciences, 1245 Lincoln Dr, Southern Illinois University at Carbondale, Carbondale, Illinois 62901, United States.

Analytical chemistry
|August 31, 2023
PubMed
概括

锁核酸 (LNA) 微探测器为检测与神经退行性疾病相关的基因扩张提供了一种新的方法. 这种方法特别识别了并列重复,克服了二次结构带来的挑战,并提高了诊断准确性.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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