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Updated: Jul 17, 2025

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使用TaqMan实时PCR技术快速检测子梅格里病毒

Jinpeng Zhang1, Huanru Fu2, Cuiteng Chen1

  • 1Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.

Poultry science
|August 31, 2023
PubMed
概括

一种新的TaqMan实时PCR方法可以准确检测子巨型病毒 (PiMeVs). 这种敏感和特定的测试证实了赛便和胚胎中的PiMeVs,这表明潜在的垂直传播.

关键词:
梅格里病毒 (Megrivirus) 是一种病毒.TaqMan-qPCR 是一个非常简单的方法.赛马子 赛马子 赛马子垂直传输的垂直传输方式

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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