大豆分离蛋白的结构和功能性质的变化:不同修改方法的影响
Lu Han1, Jinlong Li2, Yitong Jiang3
1College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
通过除化,化或糖化对大豆蛋白分离物 (SPI) 进行修改,可以提高其溶解性和乳化性能. 糖化证明是最有效的,显著改善了食品应用的SPI功能.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- 大豆蛋白分离物 (SPI) 是食品加工中的关键植物蛋白.
- 它的球形结构限制了对疏水性残留物的暴露,影响了功能性质.
研究的目的:
- 研究除化,酸化和糖化对SPI结构和功能的影响.
- 确定最优的修改来改善SPI的功能性质.
主要方法:
- 里埃变换红外光谱法 (FTIR) 是一种方法.
- 循环二重化 (CD) 光谱法 循环二重化 (CD) 光谱法
- 光光谱学是一种光谱学.
- 扫描电子显微镜 (SEM) 的使用
- 溶解度,乳化活性指数 (EAI) 和乳化稳定指数 (ESI) 的测量.
主要成果:
- 所有的修改 (除氧化,酸化,糖化) 都降低了SPI的zeta潜力.
- 糖化SPI (MSPI) 呈现出优异的溶解度 (86.73%),EAI (118.89 m2/g) 和ESI (273.33 分).
- 功能性质的改进顺序:MSPI > PSPI > DSPI. 功能性质的改进顺序:MSPI > PSPI > DSPI. 功能性质的改进顺序:MSPI > PSPI.
结论:
- 脱化,化和糖化有效修改SPI结构并增强功能性质.
- 甘化是改善SPI溶解性和乳化能力的最有前途的修改.
- 了解这些结构功能关系有助于优化植物性蛋白质成分.
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