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Updated: Jul 17, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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目标诱导的多路径对一个基质的方法,用于高效的微RNA生物分析

Wei-Guo Yang1, Hao-Ran Chen1, Ming-Li Su1

  • 1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.

Talanta
|August 31, 2023
PubMed
概括

这项研究引入了一种新的策略,用于准确检测microRNA (miRNA),使用多路径到一个基质的方法. 该方法提高了疾病诊断中的生物标志物分析的灵敏度和效率.

关键词:
同基质的消耗是同基质的消耗.核酸是核酸中的一种.实时光测定实时光测定信号放大 信号放大

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MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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