单细胞转录组学揭示了IgG4-RD中的三级淋巴体结构中的K酶表达细胞毒性Tfh细胞
Ryuichi Aoyagi1, Takashi Maehara2, Risako Koga1
1Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Fukuoka, Japan.
The Journal of allergy and clinical immunology
|August 31, 2023
概括
研究人员在IgG4相关疾病 (IgG4-RD) 组织中发现了一种新型的细胞毒性T卵泡辅助细胞 (Tfh). 毛囊T调节 (Tfr) 细胞可能会抑制这些细胞毒性Tfh细胞在受影响的器官.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 病理学 病理学 病理学
背景情况:
- 由T毛囊辅助细胞 (Tfh) 和T毛囊调节细胞 (Tfr) 协调的生殖中心 (GC) 反应,对于产生高亲和力的抗体至关重要.
- 受影响组织内的三级淋巴体器官 (TLOs) 与组织释放的抗原有关的获得免疫反应有关,其中包括GCs.
- 与免疫球蛋白G4相关的疾病 (IgG4-RD) 具有明显的异型切换和受影响组织中TLOs的存在.
研究的目的:
- 通过单细胞转录组学,识别和表征在被诊断患有IgG4-RD的患者的TLO内特定的GC-Tfh细胞子集.
- 获得IgG4-RD中组织透GC-Tfh细胞群的全面和公正的理解.
主要方法:
- 单细胞RNA测序用于从IgG4-RD患者受影响的病变中获得的排序的CD3+T细胞.
- 进行了多色免疫光分析,以调查这些病变中的CD4+CXCR5+Bcl6+GC-Tfh细胞的特征.
主要成果:
- 单细胞分析显示了5个不同的入CD4+CXCR5+Bcl6+Tfh细胞的集群.
- 在IgG4-RD患者中发现了一种新的HLA+大酶K+ (GZMK+) Tfh细胞子集,表现出细胞毒性相关的特征.
- 观察到大量的透Tfr细胞具有抑制器相关的特征,这些GZMK+Tfh细胞和Tfr细胞在受影响的组织中同定位.
结论:
- 这项研究确定了一种以前未知的HLA+GZMK+细胞毒性Tfh细胞子集,它们通过IgG4-RD透到受影响的器官.
- 这些发现表明,透Tfr细胞在IgG4-RD的背景下抑制细胞毒性Tfh细胞的活性方面具有潜在的调节作用.
更多相关视频
07:39Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Published on: January 7, 2019
12.3K
05:03Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges
Published on: April 26, 2024
718
相关概念视频
Cytotoxic T Cells-mediated Immune Response
963
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
963
T Cell Types and Functions
1.1K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.1K
T Cell Activation and Clonal Selection
804
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
804
