相关实验视频

Updated: Jul 17, 2025

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs

Published on: August 11, 2016

来自脂肪基质干细胞的外基因组通过microRNA-181a/SIRT1轴减轻痕的形成

Jie Chen¹, Wenjuan Yu², Chao Xiao²

¹Department of Plastic Surgery, The Second Affiliated Hospital of Xi'an Medical College, China; Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, China.

Archives of biochemistry and biophysics

|August 31, 2023

概括

脂肪酸衍生中酶干细胞外体通过调节microRNA-181a和SIRT1.1a来减少痕. 这一途径抑制了纤维细胞到肌纤维细胞分化和原蛋白生产的途径,为缩性痕提供了一种新的治疗方法.

科学领域:

再生医学是一种再生医学.
分子生物学分子生物学
皮肤病学皮肤病学

背景情况:

病理性痕,特别是缩性痕,在受伤后具有重大临床挑战.
来自脂肪衍生介质干细胞 (ADMSC) 的外体体显示出改善痕结果的潜力.
需要阐明ADMSC在痕调制中的外体组有效性背后的精确分子机制.

研究的目的:

调查ADMSC外基因组通过哪些潜在机制减轻过度缩性痕.
探索微RNA-181a/SIRT1轴在ADMSC外体介导痕抑制中的作用.
评估ADMSC外体在痕的临床前模型中的治疗潜力.

主要方法:

提取了ADMSC外基因组并进行了表征.
在体外研究中使用了用TGF-β1.1刺激的纤维细胞和肌纤维细胞模型.
在体内研究中使用了小鼠皮肤切口模型,干预措施包括外体注射和microRNA/SIRT1调制.

主要成果:

在小鼠伤口愈合模型中,ADMSC外体注射减少了原沉积.
微RNA-181a的表达在缩性痕组织中升高,其抑制模仿了外体效应.
确定了microRNA-181a/SIRT1通路作为一个关键的调解者,SIRT1抑制减少了外体细胞的有效性.

结论:

ADMSC外基因组通过降低微RNA-181a的调节,然后通过上调SIRT1.1来对抗病理性痕.
这个调节轴抑制纤维细胞激活和原蛋白合成,这是痕形成的关键过程.
ADMSC外生体代表了一种有前途的无细胞治疗策略,用于管理多变性痕.

关键词:

脂肪酸衍生的中介细胞干细胞.异构体是什么? 异构体是什么? 外构体是什么?过度缩的痕过度缩的痕微型RNA-181a是一种微型RNA.一个SIRT1系统.

更多相关视频

Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs

Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs

Published on: May 3, 2024

Mechanical Micronization of Lipoaspirates for Regenerative Therapy

Mechanical Micronization of Lipoaspirates for Regenerative Therapy

Published on: March 15, 2019

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...