在体内通过用屏蔽小核酸基因条形码对人类CAR T细胞命运进行多重复合的转录基因分析
Xiaoyin Lu1, Shane M Lofgren1, Yuehui Zhao2
1Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Nature biomedical engineering
|August 31, 2023
概括
一种新的方法,SSN-seq,永久标记了仿真抗原受体 (CAR) T细胞,以便在体内跟踪它们的命运. 这项技术揭示了特定的制造条件增强了持续的CD4+记忆T细胞的体内扩张,有助于采用细胞治疗的发展.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 了解仿真抗原受体 (CAR) T 细胞的体内行为对于优化采用细胞疗法至关重要.
- 需要对CAR T细胞产品进行永久标签,以便在同一微环境中同时进行分析.
研究的目的:
- 开发一种使用单细胞RNA测序 (scRNA-seq) 进行细胞体内多重纵向剖析的新型细胞条形编码方法.
- 在特定的ex vivo制造条件下研究CAR T细胞的体内动力学.
主要方法:
- 介绍了基于屏蔽的小核酸scrRNA-seq (SSN-seq),一种与3'和5'scrRNA-seq兼容的细胞条形编码技术.
- 利用Pol III U6促进体来表达小RNA条形码,用于从输液到隔离的强大的细胞身份记录.
- 在白血病的瘤再挑战小鼠模型中追踪了CAR T细胞动态.
主要成果:
- 在体内,SSN-seq成功启用了CAR T细胞的多重纵向分析.
- 现体制造条件涉及细胞因子和小分子抑制剂,促进了持续的CD4+记忆T细胞在体内扩张.
- 证明了SSN-seq在探测细胞状态动态中的实用性.
结论:
- SSN-seq是一种强大的工具,可以在体内跟踪细胞命运和动态.
- 优化的ex vivo制造策略可以增强CAR T细胞的持久性和扩张.
- SSN-seq可以促进采用细胞疗法的开发和改进.
相关概念视频
03:21RNA-seq
10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
02:24Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K