细胞外矩阵硬度通过Igfbp3/Smad途径加剧尿道狭窄
Kaixuan Li1,2,3, Ke Ding2,3, Quan Zhu2,3
1Department of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Scientific reports
|August 31, 2023
概括
增加尿道矩阵刚度驱动纤维细胞变化在狭窄. Igfbp3/Smad通路调节了这种进展,为尿道纤维化提供了潜在的治疗点.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
背景情况:
- 尿道狭窄,尿道狭窄,通常是由炎症引起的,但其进展机制尚不清楚.
- 以前的研究表明炎症起作用,但导致尿道纤维化的物理和分子因素仍然不清楚.
研究的目的:
- 调查矩阵度在尿道狭窄进展中的作用.
- 在尿道狭窄中,确定涉及性诱导的纤维细胞到肌纤维细胞过渡 (FMT) 的分子途径.
主要方法:
- 原子力显微镜 (AFM) 用于测量人类和老鼠模型中的尿道矩阵刚度.
- 聚烯胺水凝模仿了不同的矩阵刚度来研究大鼠尿道纤维细胞.
- RNA测序 (RNA-seq) 分析了基因表达变化,重点关注Igfbp3/Smad途径.
主要成果:
- 与正常组织 (5.23 kPa) 相比,尿道狭窄组织表现出明显更高的矩阵刚度 (41.59 kPa).
- 增加的矩阵刚度促进了纤维细胞到肌纤维细胞过渡 (FMT),由增强的α-SMA和原I表达证明.
- 抑制Igfbp3阻断了硬度诱导的FMT并降低了p-Smad2/3水平,而Igfbp3过度表达促进了FMT.
结论:
- 矩阵刚度是尿道狭窄的进展的一个关键因素.
- 该Igfbp3/Smad通路与调节硬度驱动的FMT和尿道纤维化有关.
- 准Igfbp3/Smad通路为尿道狭窄提供了一个有前途的治疗策略.
更多相关视频
11:51A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells
Published on: July 5, 2018
11.7K
13:18How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
Published on: September 20, 2016
9.7K
相关概念视频
01:13Cell-matrix's Response to Mechanical Forces
2.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
2.7K
01:26Extracellular Matrix
2.9K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
2.9K
01:29The Extracellular Matrix
9.1K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
9.1K
01:15Relation between Poisson's ratio, Modulus of Elasticity and Modulus of Rigidity
290
Deformation occurs in axial and transverse directions when an axial load is applied to a slender bar. This deformation impacts the cubic element within the bar, transforming it into either a rectangular parallelepiped or a rhombus, contingent on its orientation. This transformation process induces shearing strain. Axial loading elicits both shearing and normal strains. Applying an axial load instigates equal normal and shearing stresses on elements oriented at a 45° angle to the load axis.
290