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使用工程制造的Saccharomyces cerevisiae酒精K6生产S-甲基-氨酸

Jun-Min Lee¹, Min-Ho Park¹, Bu-Soo Park^1,2

¹Department of Chemical & Biological Engineering, Korea University, Seoul 136-763, Korea.

Journal of industrial microbiology & biotechnology

|August 31, 2023

概括

工程酵母现在可以生产S-甲基甲 (SMM),也被称为维生素U. 这项研究利用基因改造优化了Saccharomyces cerevisiae,以获得更高的SMM产量.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

S-甲基甲 (SMM),或维生素U,是一种有价值的植物性化合物.
微生物生产的SMM提供了一个可持续的替代植物提取.
麦芽是一种合适的宿主,因为它自然产生S-adenosylmethionine (SAM).

研究的目的:

为了设计Saccharomyces cerevisiae用于微生物生产S-甲基甲 (SMM).
通过优化代谢途径和减少降解来提高SMM产量.
为SMM合成建立一个GRAS (一般公认的安全) 微生物平台.

主要方法:

在Saccharomyces cerevisiae K6.6.中引入了Arabidopsis thaliana MMT基因.
淘汰MHT1和SAM4基因以防止SMM降解.
删除MMP1以抑制SMM进口和过度表达ACS2以增加SAM的可用性.
删除MLS1以进一步提高SAM前体水平.

主要成果:

在工程Saccharomyces cerevisiae中成功产生SMM的微生物.
通过料批发发酵实现了1.92g/L的SMM产量.
证明了向基因修改在增强SMM生产方面的有效性.

结论:

经过工程改造的Saccharomyces cerevisiae可以有效地产生S-甲基甲.
代谢工程策略显著改善了SMM收益率.
这项工作为大规模的微生物SMM生产提供了基础.

关键词:

糖果糖 (Saccharomyces cerevisiae) 糖果糖 K6 糖果糖糖果糖 K6 糖果糖甲基因因 S-甲基转移酶的方法这是一种S-adenosyl-methionine.在S-甲基-甲氨酸中,S-甲基-甲氨酸是

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