超偏磁复合纳米线与分子固定,用于超敏感的无标签蛋白质组
Shuang Zhang1,2, Behafarid Ghalandari1,2, Aiting Wang1,2
1Department of Anesthesiology and Surgical Intensive Care Unit, Xinhua Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Angewandte Chemie (International ed. in English)
|August 31, 2023
概括
带有分子的超偏磁纳米粒子增强了用于质谱的蛋白质丰富. 基于Anchor-nanoparticles for Proteomics (SWAP) 方法的简化工作流改善了微量样本中的蛋白质覆盖.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 纳米技术纳米技术
背景情况:
- 使用质谱的无标签蛋白质组学是一种关键技术.
- 微量样本分析受到预处理期间蛋白质吸附的限制.
- 现有的方法在小容量生物流体中扎着低回收率.
研究的目的:
- 开发新型超偏磁纳米粒子,以从微量生物流体中有效丰富蛋白质.
- 引入简化工作流 (SWAP) 以实现无偏见的蛋白质捕获和分析.
- 在有限的样本量中改善蛋白质组覆盖和量化.
主要方法:
- 超偏磁复合纳米粒子与分子合剂 (MGs) 的功能化.
- 基于蛋白质组学 (SWAP) 纳米粒子方法的简化工作流程的开发.
- 应用SWAP用于细胞系和人类生物流体的蛋白质组学分析.
主要成果:
- 使用纳米粒子实现了>95%的蛋白质结合和90%的恢复率.
- 使用SWAP,从100个HEK 293T细胞中量化了2500多个蛋白质组.
- 在患者研究中,仅从5μL的人体水性中鉴定出约1400种蛋白质.
结论:
- SWAP简化了样本的准备,并最大限度地减少了蛋白质的损失.
- 开发的纳米粒子丰富策略显著提高了微量样本的蛋白质覆盖范围.
- 这种方法为无标签蛋白质组学提供了更好的灵敏度和效率.
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