相关实验视频

Updated: Jul 17, 2025

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Published on: October 5, 2016

微观,转录组和IAA内容的比较分析揭示了两种种植物Ilex verticillata的茎生长变异

Sini Qin^1,2,3, Siyi Fu^1,2,3, Ying Yang^1,3

¹State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China.

Plants (Basel, Switzerland)

|September 1, 2023

概括

在Ilex verticillata品种的茎生长差异,

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
植物生理学植物生理学

背景情况:

Ilex verticillata,被称为冬,是一种受欢迎的装饰品和红杉物种.
两个不同的品种",Oosterwijk" (长茎) 和"Red sprite" (短茎),在芽生长方面表现出显著的差异.
了解这些生长变异的遗传和分子基础对于园艺应用至关重要.

研究的目的:

为了阐明"Oosterwijk"和"Red sprite"之间茎生长差异的根本原因,Ilex verticillata品种.
调查影响芽生长的微观结构,转录和荷尔蒙因素.

主要方法:

对体微观结构 (厚度,容器面积,容器数) 的比较分析.
转录组测序以识别差异表达基因 (DEGs).
印度醇-3-酸 (IAA) 含量的量测定.

主要成果:

"Oosterwijk"与"红色灵魂"相比,表现出更大的树皮厚度,血管面积和血管数量.
在烯胺生物合成,氨酸代谢和氨基酸生物合成途径中,DEGs被显著丰富.
关键基因 (例如,AST,HCT,bHLH 94) 和与auxin相关的DEGs (例如,GH3.6,IAA27) 被确定为潜在的关键对射击差异化.

结论:

"Oosterwijk"和"Red sprite"的茎生长变化与参与烯和氨基酸代谢的DEG有关.
辅酶含量和辅酶信号通路DEG在调节芽发育差异方面发挥着重要作用.
这项研究为Ilex verticillata的种类特定生长模式提供了分子洞察力.

关键词:

在IAA内容中,IAA内容包括:伊莱克斯垂直形辅助剂辅助剂辅助剂树干树干树干树干转录组 (transcriptome) 是一个转录组.在Xylem中,Xylem就是Xylem.

更多相关视频

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

Published on: March 15, 2018

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Published on: December 2, 2016

相关概念视频

Primary and Secondary Growth in Roots and Shoots

Primary and Secondary Growth in Roots and Shoots

Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.