综合全长转录组和代谢组概况揭示了黄类调节,以应对土豆中结压力
Zhiguo Zhu1, Lingling Wei1, Lei Guo1
1Yunnan Key Laboratory of Potato Biology, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, China.
Plants (Basel, Switzerland)
|September 1, 2023
概括
野生土豆的亲属由于增加的黄类积累而表现出更强的冷耐受性. 这项研究揭示了黄类生物合成途径.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 寒冷压力严重影响作物产量,特别是敏感物种,如种植土豆 (Solanum tuberosum L.).
- 野生亲属,如Solanum commersonii,具有显著的耐性,为农业改进提供了潜力.
研究的目的:
- 调查Solanum commersonii与Solanum tuberosum相比更优越的结耐受性背后的分子和代谢机制.
- 为了确定关键的基因和途径,涉及到土豆的抗反应.
主要方法:
- 对冷敏感 (DM) 和抗 (CM) 的马品种进行了比较的转录组和代谢组分析.
- 高通量测序确定了数千个转录,包括编码和非编码RNA.
- 使用了KEGG丰富分析,黄类代谢组分析,qRT-PCR和协同表达分析.
主要成果:
- 在冷压力下,耐性CM品种中,黄类生物合成途径被显著上调.
- 代谢分析证实,CM中的黄类积累量高于DM中的黄类.
- 黄类路径中的关键基因,包括合成酶 (CHS-1) 和黄类3'-基酶 (F3'H-1),以及调节基因 (Myb相关的,UGTs),显示CM中转录水平增加.
结论:
- 增强的黄酸生物合成途径和随后的糖化黄酸的积累,大大促进了在耐性马品种中观察到的结耐受性增加.
- 了解这些机制可以指导开发耐寒土豆作物的育种策略.
相关概念视频
02:45Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
02:22Introduction to Plant Diversity
44.9K
From Water to Land
44.9K
02:04Regulation of Transpiration by Stomata
28.4K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.4K
02:02Responses to Salt Stress
13.2K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.2K
02:41Responses to Drought and Flooding
10.7K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.7K
01:57Adaptations that Reduce Water Loss
25.7K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.7K