开发环保渐变色谱方法的新型实验设计范式,用于同时确定甲基和螺旋氨酸
Safa M Megahed1, Ahmed A Habib1, Sherin F Hammad1
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Journal of separation science
|September 1, 2023
概括
使用实验设计开发了一种新的绿色色染色法,用于同时分析甲基醇 (MTR) 和螺旋氨酸 (SPR). 这种强大的方法可以有效地确保药物配方中药物的精确量化.
科学领域:
- 分析化学 分析化学
- 绿色化学 绿色化学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 对于药品质量控制而言,同时对甲化 (MTR) 和螺旋 (SPR) 的分析至关重要.
- 开发环保的分析方法是制药分析中的一个越来越重要的优先事项.
研究的目的:
- 开发和优化绿色渐变色谱法,用于同时确定MTR和SPR.
- 为了验证其在药物配方中的应用而开发的方法.
主要方法:
- 利用实验设计,包括分数因数和Box-Behnken设计,用于方法选和优化.
- 使用二极管阵列探测器在C18柱上使用渐变化.
- 通过使用三种已建立的评估工具,评估了该方法的绿色性.
主要成果:
- 通过特定的移动相梯度,流速和温度实现了最佳色谱条件.
- 对于两种药物,在5-125μg/mL范围内表现出线性反应.
- 获得了MTR和SPR的低检测和量化极限,适合配方分析.
结论:
- 开发的绿色渐变色谱法是强大的,高效的,适合于同时分析MTR和SPR在平板电脑配方.
- 实验设计有助于开发有效和环保的染色学方法.
- 该方法为制药分析提供了快速可靠的方法,与绿色化学原则保持一致.
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