在料批发发酵中适应养策略可以改善糖分的输送和乙醇生产率
Yueh-Hao Ronny Hung1, Michael Chae1, Dominic Sauvageau2
1Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
Bioengineered
|September 1, 2023
概括
用于生物乙醇生产的新型养策略,使用适应的糖输送,可显著提高乙醇生产率20%左右. 这项研究提高了可再生燃料生产的发酵效率.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 工业微生物学 工业微生物学
背景情况:
- 生物乙醇是关键的可再生燃料,但发酵效率的提高滞后.
- 优化发酵操作设计对于具有成本效益的生物乙醇生产至关重要.
研究的目的:
- 为Saccharomyces cerevisiae料批发发酵开发和评估新的适应养策略.
- 提高乙醇生产率,优化生物乙醇生产期间的糖分输送.
主要方法:
- 开发了基于细胞生物质的线性和指数适应的养策略.
- 使用Saccharomyces cerevisiae进行了5L料批发发酵.
- 将适应的养策略与固定脉冲养操作进行比较.
主要成果:
- 实现了0.88g/L/h (线性) 和0.87g/L/h (指数) 的乙醇生产率,比固定养增加了20%左右.
- 达到95g/L (线性) 和104g/L (指数) 的最终乙醇度,没有剩余的葡萄糖.
- 在所有养策略中保持高乙醇产量 (~91%).
结论:
- 通过优化糖分输送,适应的养策略显著提高了乙醇生产率.
- 这些新的战略为提高工业生物乙醇生产效率和降低成本提供了一条途径.
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