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Updated: Jul 17, 2025

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

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在暴露的核心光纤微流体平台上使用异热放大方法进行无标签的DNA量化

Xuegang Li1,2, He Zhang1, Yanan Zhang1,2

  • 1College of Information Science and Engineering, Northeastern University, Shenyang, Liaoning 110819, China. zhouxue@ise.neu.edu.cn.

The Analyst
|September 1, 2023
PubMed
概括

这项研究引入了一种新的,无标签的方法,用于使用光纤传感和同热放大来检测DNA. 该技术提供了快速,灵敏和便携式的脱氧核糖核酸 (DNA) 量化,对于疾病爆发管理至关重要.

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Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye07:44

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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