开发一种快速侧流检测法,用于检测抗菌体抗体
Francisca J Grill1, Sergei Svarovsky2, Maria Gonzalez-Moa2
1School of Life Sciences, Arizona State University , Tempe, Arizona, USA.
Journal of clinical microbiology
|September 1, 2023
概括
一个新的快速测试检测到对Coccidioides真菌的抗体,这是山谷热 (VF) 的原因. 这种护理点测试可以准确地识别人类和动物的抗真菌抗体,有助于更快的VF诊断.
科学领域:
- 医学真菌学 医学真菌学
- 免疫诊断 免疫诊断 免疫诊断 免疫诊断
- 传染性疾病 传染性疾病
背景情况:
- * Coccidioides spp. 这种病原体. 这些真菌在哺乳动物中引起菌菌病 (山谷热).
- *抗体检测对于诊断和监测山谷热病至关重要.
- * 基因酶1 (CTS1) 是检测抗菌性IgG抗体的关键抗原.
研究的目的:
- * 评估一种与酶相关的间接免疫吸收试验 (ELISA),用于检测IgG到CTS1.
- * 开发和验证一种快速侧流测定 (LFA) 针对抗CTS1抗体.
- * 评估LFA在人类和非人类哺乳动物样本中的性能.
主要方法:
- *间接ELISA用于检测178个人类样本中的IgG对CTS1的抗体.
- * 开发了一种快速侧流测定 (LFA),并对143个样本进行了测试.
- *LFA性能与免疫扩散 (ID) 和补体固定 (CF) 试验进行了比较.
- *从狗,和海豚的血清/血使用CTS1 LFA进行了测试.
主要成果:
- *间接ELISA检测到92.7%的ID/CF阳性患者的抗CTS1IgG.
- *LFA表明92.9%的积极和97.7%的负面协议与ID/CF测定.
- * 在LFA上的测试线强度与已确定的ID和CF标位线性相关.
- *LFA成功检测到犬,和海豚样本中的抗CTS1抗体.
结论:
- * 开发的CTS1 LFA是一种快速 (10分钟) 临床检测方法,用于检测抗菌根抗体.
- *LFA显示与传统的血清学测定对山谷热病诊断的高度一致.
- * 这种测试可以帮助医疗保健提供者实时获得结果,提高诊断效率.
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