关联牛性疹病毒1包膜糖蛋白gD与活性-PLC-γ1(S1248)
Chang Liu1, Weifeng Yuan2, Hao Yang1
1College of Life Sciences, Hebei University , Baoding, China.
Microbiology spectrum
|September 1, 2023
概括
牛疹病毒1感染激活了脂酶C玛1 (PLC-γ1) 信号,导致其在戈尔吉装置中积累. 这种激活的PLC-γ1护送病毒颗粒释放,揭示了病毒复制中的新机制.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 已知脂酶C玛1 (PLC-γ1) 在各种细胞区内定位,包括细胞质,血膜和细胞核.
- 牛疹病毒1 (BoHV-1) 生产性感染与Ser1248 (p-PLC-γ1(S1248) 中化PLC-γ1水平的增加有关,这支持病毒复制.
研究的目的:
- 在BoHV-1感染期间调查激活的p-PLC-γ1 ((S1248) 的亚细胞局部.
- 阐明p-PLC-γ1 ((S1248) 在BoHV-1生命周期中的作用,特别是与病毒颗粒贩运有关.
主要方法:
- 同焦显微镜可视化p-PLC-γ1(S1248) 在受感染细胞中的定位.
- 同免疫沉试验用于识别与戈尔吉局部化p-PLC-γ1 ((S1248) 相互作用的蛋白质.
- 用PLC-γ1特异性抑制剂 (U73122) 治疗,以评估PLC-γ1信号在病毒输出中的功能作用.
主要成果:
- 发现激活的p-PLC-γ1 ((S1248) 在BoHV-1感染的后期阶段会在戈尔吉器官中积累.
- 戈尔吉相关的p-PLC-γ1(S1248) 特别与病毒蛋白gD结合,但不是 gC.
- 抑制PLC-γ1信号导致病毒蛋白gD在戈尔吉装置内被保留,这表明病毒外流受损.
结论:
- 这项研究确定了戈尔吉装置作为一种新型细胞区,在BoHV-1感染期间含有激活的p-PLC-γ1(S1248).
- 激活的p-PLC-γ1 ((S1248) 与细胞内和释放的病毒有关,其与gD的相互作用表明它在病毒颗粒传输中发挥作用.
- PLC-γ1信号似乎有助于从戈尔吉器官中贩运后代病毒,这代表了BoHV-1生产性感染的新机制.
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