增强悬浮细胞转移,通过诱导在暴露于电机械刺激之前,膜性行为皮层的局部分布来促进悬浮细胞转移
Wenjing Huang1,2, Yibo Ma1, Naotomo Tottori1
1Department of Mechanical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Biotechnology letters
|September 1, 2023
概括
破坏细胞的细胞.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 物理转染方法,如机电刺激,对于将分子引入细胞至关重要.
- 细胞膜下方的密集的活性蛋白层 - - 活性蛋白皮层,可能会影响转化效率.
研究的目的:
- 为了研究是否扰乱行为皮质可以提高细胞传染效率.
- 了解Actin皮质在电机械细胞刺激中的作用.
主要方法:
- 使用泡发生器对悬浮细胞进行机电刺激.
- 采用细胞素D,一种actin聚合抑制剂,在刺激之前破坏actin皮质.
- 在NIH/3T3和UMR-106细胞中评估了各种等离子体大小的转染效率.
主要成果:
- 仅仅是电机械刺激就导致了整体动因皮层信号的减少.
- 细胞素D治疗导致局部F-actin聚合和刺激后部分actin皮质破坏.
- 用细胞素D进行预处理显著提高了大型等离子体 (4.7-11kbp) 的转染效率.
结论:
- 局部化的行为皮层分布对于提高转染效率至关重要.
- 行为皮层的部分干扰改善了大型塑体进入细胞的传递.
- 准actin皮质是优化物理转染技术的一个有希望的策略.
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