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Updated: Jul 17, 2025

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传统的试管婴儿在植入前基因测试中是可行的,以检测体积

Shuoping Zhang¹, Pingyuan Xie^2,3, Fang Lan²

¹Clinical Research Center for Reproduction and Genetics in Hunan Province, Reproductive and Genetic Hospital of CITIC-Xiangya, No. 567, Tongzipo West Road, Yuelu District, Changsha, 410008, Hunan, China.

Journal of assisted reproduction and genetics

|September 1, 2023

概括

传统的体外受精 (cIVF) 对于非男性因子不孕症的夫妇是可行的,这些夫妇接受了前植入前遗传测试,以检测形状 (PGT-A). 这种方法显示出父母污染风险较低,胚胎结果与细胞体内精子注射 (ICSI) 相似.

科学领域:

生殖医学和遗传学
辅助生殖技术辅助生殖技术
基因组分析基因组分析

背景情况:

在优化试管婴儿结果方面,对动脉增生病 (PGT-A) 的植入前遗传检测至关重要.
传统的体外受精 (cIVF) 通常用于非男性因子不孕症病例.
应用cIVF与PGT-A一起需要仔细评估精子DNA放大和污染风险.

研究的目的:

评估使用cIVF对非男性因子不孕症接受PGT-A的夫妇的可行性.
评估精子全基因组放大 (WGA) 协议的PGT-A应用程序.
在PGT-A周期中,比较cIVF和细胞内精子注射 (ICSI) 之间的临床和染色体结局.

主要方法:

测试了三个精子WGA协议 (Picoplex,ChromInst,MDA) 的效率.
对641个PGT-A周期的回顾性分析,比较cIVF和ICSI的结果.
对26对接受cIVF-PGT-A的夫妇进行前性研究,包括父母污染分析和用捐赠的无胚胎胚胎进行验证.

主要成果:

多重位移放大 (MDA) 是唯一成功的精子WGA协议.
在cIVF-PGT-A和ICSI-PGT-A组之间没有发现euploid,mosaic或aneuploid胚胎率的显著差异.
前性研究在150个样本中没有发现父感染和一个母感染病例;PGT-A验证显示91.7%的准确性.

结论:

MDA WGA协议适用于cIVF-PGT-A循环,具有较低的父母污染风险.
传统的体外受精 (cIVF) 是对经过PGT-A.A.的非男性因子不孕症夫妇的可行的选择.
这一发现支持cIVF在PGT-A治疗方案中的更广泛应用.

关键词:

传统的试管婴儿是常规的胚胎是平的胚胎是平的在PGT-A中.父母污染的污染情况

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